Simple enrichment and analysis of plasma lysophosphatidic acids
Literature Information
Publication Date
2013-09-26
DOI
10.1039/C3AN01168B
Impact Factor
4.616
Authors
Jialu Wang, Martha Sibrian-Vazquez, Jorge O. Escobedo, Mark Lowry, Lei Wang, Yu-Hsuan Chu, Richard G. Moore, Robert M. Strongin
View Original
Abstract
A simple and highly efficient technique for the analysis of lysophosphatidic acid (LPA) subspecies in human plasma is described. The streamlined sample preparation protocol furnishes the five major LPA subspecies with excellent recoveries. Extensive analysis of the enriched sample reveals only trace levels of other phospholipids. This level of purity not only improves MS analyses, but enables HPLC post-column detection in the visible region with a commercially available fluorescent phospholipids probe. Human plasma samples from different donors were analyzed using the above method and validated by LC-ESI/MS/MS.
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Source Journal
Analyst
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Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.
Recommended Compounds
Benzyl 6-oxo-3,6-dihydro-1(2H)-pyridinecarboxylate
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725746-35-0
Molecular Formula:
C13H13NO3
![N-{[(2-Methyl-2-propanyl)oxy]carbonyl}-L-methionylglycine structure](https://static.chemtradehub.com/structs/234/23446-03-9-e1e5.webp "N-{[(2-Methyl-2-propanyl)oxy]carbonyl}-L-methionylglycine structure")
N-{[(2-Methyl-2-propanyl)oxy]carbonyl}-L-methionylglycine
CAS Number:
23446-03-9
Molecular Formula:
C12H22N2O5S
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