2-Methoxy-3-(1-methyl-1H-1,2,4-triazol-3-yl)aniline
CAS Number:
1609394-10-6
Molecular Formula:
C10H12N4O
Sequence-specific electrochemical detection of Alicyclobacillus acidoterrestrisDNA using electroconductive polymer-modified fluorine tin oxide electrodes
Literature Information
Publication Date
2008-10-29
DOI
10.1039/B809080G
Impact Factor
4.616
Authors
Katlin Ivon Barrios Eguiluz, Giancarlo Richard Salazar-Banda, Maribel Elizabeth Funes-Huacca, Juliana Vieira Alberice, Emanuel Carrilho, Sergio Antonio Spinola Machado, Luis Alberto Avaca
View Original
Abstract
This study outlines the quantification of low levels of Alicyclobacillus acidoterrestris in pure cultures, since this bacterium is not inactivated by pasteurization and may remain in industrialized foods and beverages. Electroconductive polymer-modified fluorine tin oxide (FTO) electrodes and multiple nanoparticle labels were used for biosensing. The detection ofA. acidoterrestris in pure cultures was performed by reverse transcription polymerase chain reaction (RT-PCR) and the sensitivity was further increased by asymmetric nested RT-PCR using electrochemical detection for quantification of the amplicon. The quantification of nested RT-PCR products by Ag/Au-based electrochemical detection was able to detect 2 colony forming units per mL (CFU mL−1) of spores in pure culture and low detection and quantification limits (7.07 and 23.6 nM, respectively) were obtained for the target A. acidoterrestris on the electrochemical detection bioassay.
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Source Journal
Analyst
CiteScore:
7.8
Self-citation Rate:
5.6%
Articles per Year:
653
Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.
Recommended Compounds
N,N'-(1R,2R)-1,2-Cyclohexanediyldi(2-pyridinecarboxamide)
CAS Number:
218290-24-5
Molecular Formula:
C18H20N4O2
![(3R)-4-(4-Chlorophenyl)-3-({[(2-methyl-2-propanyl)oxy]carbonyl}amino)butanoic acid structure](https://static.chemtradehub.com/structs/218/218608-96-9-f871.webp "(3R)-4-(4-Chlorophenyl)-3-({[(2-methyl-2-propanyl)oxy]carbonyl}amino)butanoic acid structure")
(3R)-4-(4-Chlorophenyl)-3-({[(2-methyl-2-propanyl)oxy]carbonyl}amino)butanoic acid
CAS Number:
218608-96-9
Molecular Formula:
C15H20ClNO4
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