Construction of a cleavable linker chemistry-based HBEXO-Chip to isolate circulating exosomes for breast cancer diagnosis

Literature Information

Publication Date 2023-12-06
DOI 10.1039/D3AY01938A
Impact Factor 2.896
Authors

Shanshan Zhou, Zongxin Li, Yan Li, Xiaoyao Wang, Kun Deng


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Abstract

Breast cancer is presently the most common form of malignant tumour globally, and its precise diagnosis is vital for enhancing patient survival rates and their quality of life. Exosomes, which are small extracellular vesicles containing proteins and nucleic acid molecules, have emerged as ideal cancer markers for liquid biopsy-based diagnostics. Nevertheless, the current methods for isolating exosomes present challenges for clinical implementation. Although immunoaffinity-based microfluidics hold potential for exosome-based cancer diagnostics, existing microfluidic chips struggle to capture and release intact, high-purity, and highly specific exosomes effectively. To surmount these obstacles, we developed the HBEXO-Chip, an innovative immunoaffinity microfluidic device that employs cleavable linker chemistry technology. This chip enables rapid isolation and detection of breast cancer-derived exosomes in peripheral blood. The fishbone-like microfluidic chip design of the HBEXO-Chip heightens the binding likelihood between specific exosomes and antibodies, significantly augmenting capture efficiency. Furthermore, the gentle reaction conditions of the cleavable linker chemistry retain the exosomes' membrane structure's integrity during the release process, which is advantageous for downstream experimental analysis. Our study demonstrated the effectiveness of the HBEXO-Chip in distinguishing breast cancer patients, patients with benign breast tumours, and healthy controls. By quantitatively analysing Epcam+ exosomes in clinical plasma samples, this technology platform provides a quick, user-friendly, highly sensitive, and specific assay for detecting tumour exosomes in peripheral blood, making it a valuable liquid biopsy tool for clinicians to diagnose breast cancer.

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Analytical Methods

Analytical Methods
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Analytical Methods welcomes early applications of new analytical and bioanalytical methods and technology demonstrating the potential for societal impact. We require that methods and technology reported in the journal are sufficiently innovative, robust, accurate, and compared to other available methods for the intended application. Developments with interdisciplinary approaches are particularly welcome. Systems should be proven with suitably complex and analytically challenging samples. We encourage developments within, but not limited to, the following technologies and applications: global health, point-of-care and molecular diagnostics biosensors and bioengineering drug development and pharmaceutical analysis applied microfluidics and nanotechnology omics studies, such as proteomics, metabolomics or glycomics environmental, agricultural and food science neuroscience biochemical and clinical analysis forensic analysis industrial process and method development

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