Identification of major histocompatibility complex class II-associated peptides derived from freshly prepared rat Langerhans cells using MALDI-PSD and Edman degradation

Literature Information

Publication Date 2000-03-21
DOI 10.1039/A909229C
Impact Factor 4.616
Authors

Albert Sickmann, Martin Blüggel, Michael Kulke, Holger Kremmin, Alexandra Marx, Konrad Reske, Helmut E. Meyer


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Abstract

The isolation and identification is described of MHC class II-bound peptides derived from Langerhans cells. A combination of preparative micro-HPLC, MALDI-MS, Edman degradation was used for determining the amino acid sequence of MHC-associated peptides. Sample handling was crucial because fractions containing trace amounts of material require immediate storage at −80 °C to prevent peptide losses.

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