Fluo-3AM | CAS No. 121714-22-5

Fluo-3AM

Basic Information

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CAS Number

121714-22-5

Molecular Formula

C51H50Cl2N2O23

Molecular Weight

1129.8 g/mol

Chemical Classification

Basic Chemical Raw Materials Organic Chemicals Carboxylic Acids

Basic Chemical Raw Materials Organic Chemicals Esters

Basic Chemical Raw Materials Organic Chemicals Ethers

Basic Chemical Raw Materials Organic Chemicals Heterocyclic Compounds

Pharmaceutical Chemicals Pharmaceutical Intermediates Heterocyclic Intermediates

Fine Chemicals Dyes and Pigments Organic Dyes

Fine Chemicals Surfactants Anionic Surfactants

Agricultural Chemicals Pesticides Herbicides

Laboratory Chemicals Research Chemicals Biochemical Reagents

Basic Physical Properties

Melting Point

>250℃

Boiling Point

1075.8±65.0 °C at 760 mmHg

Density

1.3080

Solubility

DMSO: soluble

SMILES

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CC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC2=C(C=CC(=C2)C3=C4C=C(C(=O)C=C4OC5=CC(=C(C=C53)Cl)OCOC(=O)C)Cl)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C

InChI

InChI=1S/C51H50Cl2N2O23/c1-28-7-9-39(54(19-47(62)74-24-69-30(3)57)20-48(63)75-25-70-31(4)58)45(13-28)66-11-12-67-46-14-34(8-10-40(46)55(21-49(64)76-26-71-32(5)59)22-50(65)77-27-72-33(6)60)51-35-15-37(52)41(61)17-42(35)78-43-18-44(38(53)16-36(43)51)73-23-68-29(2)56/h7-10,13-18H,11-12,19-27H2,1-6H3

InChIKey

PTPUOMXKXCCSEN-UHFFFAOYSA-N

LogP

5.405220000000004

Topological Polar Surface Area

301.08 Å²

Hydrogen Bond Donor/Acceptor

0 / 25

Complexity

2220

Safety Information

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Sensitiveness

sensitive to humidity

Synonyms & References

English

Fluo-3AM
Fluo-3, AM
SCHEMBL16364726
CS-3524
CHEMBL2074907
HY-D0716
N-[4-[6-[(Acetyloxy)methoxy]-2,7-dichloro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxoethyl]glycine (Acetyloxy)methyl Ester
AKOS030632503
Glycine, N-[4-[6-[(acetyloxy)methoxy]-2,7-dichloro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-, (acetyloxy)methyl ester
C51H50Cl2N2O23
F85082
AS-78723
121714-22-5
FLUO 3/AM
HB0772
Fluo 3-AM
4-(6-Acetoxymethoxy-2,7-dichloro-3-oxo-9-xanthenyl)-4'-methyl-2,2'-(ethylenedioxy)dianiline-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl) ester
acetyloxymethyl 2-[N-[2-(acetyloxymethoxy)-2-oxoethyl]-2-[2-[2-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]-5-(2,7-dichloro-3-hydroxy-6-oxoxanthen-9-yl)phenoxy]ethoxy]-4-methylanilino]acetate
Fluo 3-AM Special Packaging
Fluo-3, AM ester
Fluo-3-Acetoxymethyl ester
Fluo-3-pentaacetoxymethyl ester
4-(6-Acetoxymethoxy-2,7-dichloro-3-oxo-9-xanthenyl)-4'-methyl-2,2'(ethylenedioxy)dianiline-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl) ester
FLUO 3
4-(6-Acetoxymethoxy-2,7-dichloro-3-oxo-9-xanthenyl)-4′-methyl-2,2′(ethylenedioxy)dianiline-N,N,N′,N′-tetraacetic acid tetrakis(acetoxymethyl) ester
Fluo-8
Rhod-4
FIuo,3-AMF
Fluo 3-AM solution
FLUO 3-AM, FOR FLUORESCENCE
FLUO-3, AM

MDL Number

MFCD00083336

FAQ

What are the physical and chemical properties of Fluo-3AM (CAS: 121714-22-5)?

Fluo-3AM is a fluorescent calcium indicator dye with a molecular weight of approximately 556.37 g/mol. It exists in a crystalline form with excellent solubility in common organic solvents like DMSO and poor solubility in water. Fluo-3AM is relatively stable under physiological conditions but exhibits high reactivity in acidic environments. It is commonly used for calcium ion detection in biological systems due to its high specificity and sensitivity.

How is Fluo-3AM (CAS: 121714-22-5) typically synthesized?

Fluo-3AM is synthesized through a multi-step process involving the coupling of fluo-3 and succinimidyl ester derivatives. The synthesis typically requires the use of a coupling agent like N,N′-dicyclohexylcarbodiimide (DCC) and pyridine. The yield is generally around 70-80%, and the reaction is monitored by thin-layer chromatography (TLC) to ensure purity and selectivity.

What industries use Fluo-3AM (CAS: 121714-22-5)?

Fluo-3AM is widely used in the pharmaceutical, biotechnology, and bioresearch industries. It is essential for calcium ion detection in cell biology and biomedical research. Additionally, it is utilized in polymer science for sensing applications and in semiconductor industries for quality control in the production of electronic devices.

What precautions should be taken when handling Fluo-3AM (CAS: 121714-22-5)?

When handling Fluo-3AM, it is crucial to wear appropriate personal protective equipment (PPE) including gloves and safety goggles. Work should be conducted in a well-ventilated area or a fume hood to avoid inhalation. Spills should be immediately cleaned up with absorbent material and discarded according to local regulations. Always consult the Safety Data Sheet (SDS) for detailed handling and disposal instructions.