Compound Q&A

How is Fluo-3AM (CAS: 121714-22-5) typically synthesized?

Answer

Fluo-3AM
Fluo-3AM is synthesized through a multi-step process involving the coupling of fluo-3 and succinimidyl ester derivatives. The synthesis typically requires the use of a coupling agent like N,N′-dicyclohexylcarbodiimide (DCC) and pyridine. The yield is generally around 70-80%, and the reaction is monitored by thin-layer chromatography (TLC) to ensure purity and selectivity.

About

English Name Fluo-3AM
Molecular Formula C51H50Cl2N2O23
Molecular Weight 1129.8 g/mol
SMILES CC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC2=C(C=CC(=C2)C3=C4C=C(C(=O)C=C4OC5=CC(=C(C=C53)Cl)OCOC(=O)C)Cl)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C
InChIKey PTPUOMXKXCCSEN-UHFFFAOYSA-N
Last Updated: 2025-10-19 13:36

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