Fluo-3AM(CAS: 121714-22-5)

Fluo-3AM is a fluorescent calcium indicator dye with a molecular weight of approximately 556.37 g/mol. It exists in a crystalline form with excellent solubility in common organic solvents like DMSO and poor solubility in water. Fluo-3AM is relatively stable under physiological conditions but exhibits high reactivity in acidic environments. It is commonly used for calcium ion detection in biological systems due to its high specificity and sensitivity.

Fluo-3AM is synthesized through a multi-step process involving the coupling of fluo-3 and succinimidyl ester derivatives. The synthesis typically requires the use of a coupling agent like N,N′-dicyclohexylcarbodiimide (DCC) and pyridine. The yield is generally around 70-80%, and the reaction is monitored by thin-layer chromatography (TLC) to ensure purity and selectivity.

Fluo-3AM is widely used in the pharmaceutical, biotechnology, and bioresearch industries. It is essential for calcium ion detection in cell biology and biomedical research. Additionally, it is utilized in polymer science for sensing applications and in semiconductor industries for quality control in the production of electronic devices.

When handling Fluo-3AM, it is crucial to wear appropriate personal protective equipment (PPE) including gloves and safety goggles. Work should be conducted in a well-ventilated area or a fume hood to avoid inhalation. Spills should be immediately cleaned up with absorbent material and discarded according to local regulations. Always consult the Safety Data Sheet (SDS) for detailed handling and disposal instructions.