Mapping phosphate modifications of substituted lipid A via a targeted MS3 CID/UVPD strategy
Literature Information
Christopher M. Crittenden, Carmen M. Herrera, Peggy E. Williams, Dante P. Ricci, Lee R. Swem, M. Stephen Trent, Jennifer S. Brodbelt
Structural characterization of lipid A from Gram-negative bacteria remains a significant challenge, especially with respect to localizing modifications of the phosphate groups typically found on the reducing and non-reducing ends of the β-1′,6-linked glucosamine disaccharide backbone of lipid A. As reported here, combining traditional collisional activated dissociation (CAD) and ultraviolet photodissociation (UVPD) in a hybrid MS3 approach facilitates identification and localization of substituents of the phosphate groups. The focus is on rapid identification and characterization of substituted lipid A species with specific emphasis on the modifications on the 1 and 4′ phosphate moieties. Mapping these modifications, typically ones that modify the surface charges of lipopolysaccharides, is particularly important owing to the impact of these types of modifications on antibiotic resistance. The presence of phosphoethanolamine, aminoarabinose, and galactosamine moieties in hexaacylated and heptaacylated lipid A species, including ones from Enterobacter cloacae and Acinetobacter baumannii, are characterized using a targeted MS3 strategy to identify glycosidic product ions (1,5X1 and 0,4A2, typically) which allow localization of the substituents.
Related Literature
Insights from QM/MM-ONIOM calculations: the TADF phenomenon of phenanthro[9,10-d]imidazole-anthraquinone in the solid state
Huixue Li, Xiaofeng Wang, Kun Yuan, Lingling Lv, Zhifeng Li
DOI: 10.1039/D1CP00578B
QM calculations predict the energetics and infrared spectra of transient glutamine isomers in LOV photoreceptors
Prokopis C. Andrikopoulos, Aditya S. Chaudhari, Yingliang Liu, Patrick E. Konold, John T. M. Kennis, Bohdan Schneider, Gustavo Fuertes
DOI: 10.1039/D1CP00447F
Prediction of the standard potentials for one-electron oxidation of N,N,N′,N′ tetrasubstituted p-phenylenediamines by calculation
Cecilie L. Andersen, Evanildo G. Lacerda, Jr, Jørn B. Christensen, Stephan P. A. Sauer, Ole Hammerich
DOI: 10.1039/D1CP02315B
Review on physical impedance models in modern battery research
Rohit Ranganathan Gaddam, Leon Katzenmeier, Xaver Lamprecht, Aliaksandr S. Bandarenka
DOI: 10.1039/D1CP00673H
Correction: Thermoelectric properties of CZTS thin films: effect of Cu–Zn disorder
E. Isotta, N. Ataollahi, A. Chiappini, C. Malerba, S. Luong, V. Trifiletti, O. Fenwick, P. Scardi
DOI: 10.1039/D1CP90124A
Dynamics of photodissociation of nitric oxide from S-nitrosylated cysteine and N-acetylated cysteine derivatives in water
Hojeong Yoon, Seongchul Park, Manho Lim
DOI: 10.1039/D1CP01743H
Investigation of the thermal decomposition mechanism of glycerol: the combination of a theoretical study based on the Minnesota functional and experimental support
Dongdong Zhang, Yi Cao, Pan Zhang, Jiankang Liang, Ke Xue, Yong Xia, Zhengjian Qi
DOI: 10.1039/D1CP01526E
Thermo-osmotic pressure and resistance to mass transport in a vapor-gap membrane
Michael T. Rauter, Sondre K. Schnell, Bjørn Hafskjold, Signe Kjelstrup
DOI: 10.1039/D0CP06556K
Correction: De novo prediction of cross-effect efficiency for magic angle spinning dynamic nuclear polarization
Anne-Laure Barra, Johan van Tol, Sabine Hediger, Daniel Lee, Gaël De Paëpe
DOI: 10.1039/D1CP90117F
You might also like
What is the market or research trend for N-(4-Methoxybenzyl)-2-pyridinamine (CAS: 52818-63-0)?
N-(4-Methoxybenzyl)-2-pyridinamine (CAS: 52818-63-0) is increasingly being used ...
What precautions should be taken when handling Ethyl 4-(2-chlorophenyl)-1,3-thiazole-2-carboxylate (CAS: 1050507-06-6)?
When handling Ethyl 4-(2-chlorophenyl)-1,3-thiazole-2-carboxylate, appropriate p...
What regulatory guidelines apply to diethyldiselane (CAS: 628-39-7)?
Diethyldiselane (CAS: 628-39-7) is classified under the Globally Harmonized Syst...
What is the market or research trend for oxocopper (CAS: 12053-18-8)?
The market for oxocopper (CAS: 12053-18-8) is primarily driven by its use in cat...
What is the market or research trend for 5-{[(2-Methyl-2-propanyl)oxy]carbonyl}-5-azaspiro[2.4]heptane-7-carboxylic acid?
The market for 5-{[(2-Methyl-2-propanyl)oxy]carbonyl}-5-azaspiro[2.4]heptane-7-c...
What is 2-(1-Pyrrolidinyl)-4-pyridinamine (CAS: 35981-63-6)?
2-(1-Pyrrolidinyl)-4-pyridinamine is a chemical compound with the CAS number 359...
What are the physical and chemical properties of 2-(3-Pyridinyl)-1-azabicyclo[2.2.2]octane (CAS: 91556-75-1)?
2-(3-Pyridinyl)-1-azabicyclo[2.2.2]octane (CAS: 91556-75-1) is a crystalline sol...
How is (S)-Alpha-allyl-proline hydrochloride (CAS: 129704-91-2) typically synthesized?
(S)-Alpha-allyl-proline hydrochloride is usually synthesized via a Wittig reacti...
What is 3-Methyl-1,2-oxazole-5-carboxylic acid (CAS: 4857-42-5)?
3-Methyl-1,2-oxazole-5-carboxylic acid (CAS: 4857-42-5) is an organic compound w...
How is Lys-SMCC-DM1 (CAS: 1281816-04-3) typically synthesized?
Lys-SMCC-DM1 is synthesized via a multi-step process involving the coupling of S...
Source Journal
Analyst

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.













![N-{[(2-Methyl-2-propanyl)oxy]carbonyl}-L-methionylglycine structure N-{[(2-Methyl-2-propanyl)oxy]carbonyl}-L-methionylglycine structure](https://static.chemtradehub.com/structs/234/23446-03-9-e1e5.webp)