FTIR study on the light sensitivity of the [NiFe] hydrogenase from Desulfovibrio vulgaris Miyazaki F: Ni–C to Ni–L photoconversion, kinetics of proton rebinding and H/D isotope effect
Literature Information
Maria-Eirini Pandelia, Leslie J. Currell, Helmut Görner, Wolfgang Lubitz
The light-induced Ni–C to Ni–L transition results in the dissociation of a hydrogenic species, originating from the dihydrogen splitting at the active site. Back conversion in the dark to form Ni–C was investigated by studying the rebinding kinetics of this ligand in protonated (H2/H2O) and deuterated (D2/D2O) samples using time resolved FTIR spectroscopy.
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