5-Aminofluorescein

Basic Information

View in Editor

CAS Number

3326-34-9

Molecular Formula

C20H13NO5

Molecular Weight

347.33 g/mol

Chemical Classification

Basic Chemical Raw Materials Organic Chemicals Carboxylic Acids

Basic Chemical Raw Materials Organic Chemicals Esters

Basic Chemical Raw Materials Organic Chemicals Ethers

Basic Chemical Raw Materials Organic Chemicals Amines

Basic Chemical Raw Materials Organic Chemicals Heterocyclic Compounds

Pharmaceutical Chemicals Pharmaceutical Intermediates Heterocyclic Intermediates

Fine Chemicals Dyes and Pigments Organic Dyes

Fine Chemicals Surfactants Anionic Surfactants

Basic Physical Properties

Physical State

Powder

Melting Point

223 ºC

Boiling Point

695.3 ℃ at 760 mmHg

Water Solubility

Soluble in water (partly), methanol (10 mg/ml), and acetone.

Refractive Index

1.5180 (estimate)

SMILES

View in Editor

Nc1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc53

InChI

InChI=1S/C20H13NO5/c21-10-1-4-14-13(7-10)19(24)26-20(14)15-5-2-11(22)8-17(15)25-18-9-12(23)3-6-16(18)20/h1-9,22-23H,21H2

InChIKey

GZAJOEGTZDUSKS-UHFFFAOYSA-N

LogP

3.248000000000001

Topological Polar Surface Area

102.01000000000002 Å²

Hydrogen Bond Donor/Acceptor

4 / 6

Complexity

557

Safety Information

View Safety Information

GHS Hazard Pictograms

H302

H315

H319

H335

Hazard Statement

H302 Harmful if swallowed
Acute toxicity, oral

H315 Causes skin irritation
Skin corrosion/irritation

H319 Causes serious eye irritation
Serious eye damage/eye irritation

H335 May cause respiratory irritation
Specific target organ toxicity, single exposure; Respiratory tract irritation

Stability

Light and Moisture Sensitive

Sensitiveness

易吸潮,对热敏感

Synonyms & References

English

5-Aminofluorescein
5-Amino-2-(3-hydroxy-6-oxoxanthen-9-yl)benzoic acid
5-Aminofluorescein (isomer I)
4-Aminofluorescein
5-AF
Fluoresceinamine, Isomer 1
Fluoresceinamine, isomer I
5-amino-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid
5-amino-6'-hydroxy-3H-spiro[2-benzofuran-1,9'-xanthene]-3,3'(9a'H)-dione
Fluoresceinamine isomer I, pure
5-Amino-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-(9H)xanthen]-3-one (isomer I)
Fluoresceinamine (isomer I)

MDL Number

MFCD00005052

FAQ

What are the physical and chemical properties of 5-Aminofluorescein (CAS: 3326-34-9)?

5-Aminofluorescein (CAS: 3326-34-9) is a fluorescent dye with a molecular weight of approximately 275.29 g/mol. It exists in a crystalline form and is soluble in water and most organic solvents. The dye is highly reactive towards nucleophiles and exhibits excellent fluorescence properties, making it suitable for various analytical techniques.

How is 5-Aminofluorescein (CAS: 3326-34-9) typically synthesized?

5-Aminofluorescein (CAS: 3326-34-9) is synthesized by the reaction of 3-hydroxy-5-nitrofluorescein with an amine. Commonly, this involves the reduction of the nitro group followed by an alkylation step to introduce the amine functionality. The reaction is typically carried out in the presence of a catalyst such as sodium hydride, and the yield is usually around 70-80%.

What industries use 5-Aminofluorescein (CAS: 3326-34-9)?

5-Aminofluorescein (CAS: 3326-34-9) is extensively used in the pharmaceutical industry for drug screening and analysis, in the polymer industry for labeling and identification, in the sensor industry for detection of specific analytes, and in the semiconductor industry for quality control and process monitoring.

What precautions should be taken when handling 5-Aminofluorescein (CAS: 3326-34-9)?

When handling 5-Aminofluorescein (CAS: 3326-34-9), it is important to wear appropriate personal protective equipment (PPE), including gloves, goggles, and a lab coat. Work should be conducted in a well-ventilated area or a fume hood. Spills should be handled according to the Material Safety Data Sheet (MSDS) guidelines. Proper storage should be in a cool, dry place away from direct sunlight and heat sources.