5-Aminofluorescein(CAS: 3326-34-9)

5-Aminofluorescein (CAS: 3326-34-9) is a fluorescent dye with a molecular weight of approximately 275.29 g/mol. It exists in a crystalline form and is soluble in water and most organic solvents. The dye is highly reactive towards nucleophiles and exhibits excellent fluorescence properties, making it suitable for various analytical techniques.

5-Aminofluorescein (CAS: 3326-34-9) is synthesized by the reaction of 3-hydroxy-5-nitrofluorescein with an amine. Commonly, this involves the reduction of the nitro group followed by an alkylation step to introduce the amine functionality. The reaction is typically carried out in the presence of a catalyst such as sodium hydride, and the yield is usually around 70-80%.

5-Aminofluorescein (CAS: 3326-34-9) is extensively used in the pharmaceutical industry for drug screening and analysis, in the polymer industry for labeling and identification, in the sensor industry for detection of specific analytes, and in the semiconductor industry for quality control and process monitoring.

When handling 5-Aminofluorescein (CAS: 3326-34-9), it is important to wear appropriate personal protective equipment (PPE), including gloves, goggles, and a lab coat. Work should be conducted in a well-ventilated area or a fume hood. Spills should be handled according to the Material Safety Data Sheet (MSDS) guidelines. Proper storage should be in a cool, dry place away from direct sunlight and heat sources.