DNA nanolantern-based split aptamer probes for in situ ATP imaging in living cells and lighting up mitochondria‡
Literature Information
Ya-Xin Wang, Dong-Xia Wang, Jia-Yi Ma, Jing Wang, Yi-Chen Du, De-Ming Kong
Accurate and specific analysis of adenosine triphosphate (ATP) expression levels in living cells can provide valuable information for understanding cell metabolism, physiological activities and pathologic mechanisms. Herein, DNA nanolantern-based split aptamer nanoprobes are prepared and demonstrated to work well for in situ analysis of ATP expression in living cells. The nanoprobes, which carry multiple split aptamer units on the surface, are easily and inexpensively prepared by a “one-pot” assembly reaction of four short oligonucleotide strands. A series of characterization experiments verify that the nanoprobes have good monodispersity, strong biostability, high cell internalization efficiency, and fluorescence resonance energy transfer (FRET)-based ratiometric response to ATP in the concentration range covering the entire intracellular ATP expression level. By changing the intracellular ATP level via different treatments, the nanoprobes are demonstrated to show excellent performance in intracellular ATP expression analysis, giving a highly ATP concentration-dependent ratiometric fluorescence signal output. ATP-induced formation of large-sized DNA aggregates not only amplifies the FRET signal output, but also makes in situ ATP-imaging analysis in living cells possible. In situ responsive crosslinking of nanoprobes also makes them capable of lighting up the mitochondria of living cells. By simply changing the split aptamer sequence, the proposed DNA nanolantern-based split aptamer strategy might be easily extended to other targets.
Related Literature
Ring selectivity and migratory aptitude of Cp*Ru+ complexation to acecorannulene
T. Jon Seiders, Joseph M. O'Connor
DOI: 10.1039/B316061K
Tetracyanoresorcin[4]arene ion channel shows pH dependent conductivity change
Song-De Tan, Mika Yamamura
DOI: 10.1039/B312952G
Porphyrin ring contraction: a one-pot reaction leading to divalent corroles
Christophe Jeandon, Romain Ruppert, Henry J. Callot
DOI: 10.1039/B400812J
Significant promotional effect of CCl4 on fullerene yield in the graphite arc-discharge reaction
Fei Gao, Su-Yuan Xie, Rong-Bin Huang, Lan-Sun Zheng
DOI: 10.1039/B306921B
DPA-substituted coumarins as chemosensors for zinc(ii): modulation of the chemosensory characteristics by variation of the position of the chelate on the coumarin
Nathaniel C. Lim, Christian Brückner
DOI: 10.1039/B403448A
Fluorescence studies of protein thermostability in ionic liquids
Sheila N. Baker, T. Mark McCleskey, Siddharth Pandey, Gary A. Baker
DOI: 10.1039/B401304M
Coordinative and electrostatic forces in action: from the design of differential chromogenic anion sensors to selective carboxylate recognition
Beatriz García-Acosta, Xavier Albiach-Martí, Eduardo García, Luis Gil, Ramón Martínez-Máñez, Knut Rurack, Félix Sancenón, Juan Soto
DOI: 10.1039/B314997H
Electrochemical lithography: fabrication of nanoscale Si tips by porous anodization of Al/Si wafer
L. Pu, Y. Shi, J. M. Zhu, X. M. Bao, R. Zhang, Y. D. Zheng
DOI: 10.1039/B315810A
Synthesis of nearly monodisperse polystyrene–polypeptideblock copolymersviapolymerisation of N-carboxyanhydrides
Ivaylo Dimitrov, Helmut Schlaad
DOI: 10.1039/B308990H
Regioselective uncatalysed hydrophosphination of alkenes: a facile route to P-alkylated phosphine derivatives
Olivier Delacroix, Annie-Claude Gaumont
DOI: 10.1039/B311892D
You might also like
What is the market or research trend for N-(4-Methoxybenzyl)-2-pyridinamine (CAS: 52818-63-0)?
N-(4-Methoxybenzyl)-2-pyridinamine (CAS: 52818-63-0) is increasingly being used ...
What precautions should be taken when handling Ethyl 4-(2-chlorophenyl)-1,3-thiazole-2-carboxylate (CAS: 1050507-06-6)?
When handling Ethyl 4-(2-chlorophenyl)-1,3-thiazole-2-carboxylate, appropriate p...
What regulatory guidelines apply to diethyldiselane (CAS: 628-39-7)?
Diethyldiselane (CAS: 628-39-7) is classified under the Globally Harmonized Syst...
What is the market or research trend for oxocopper (CAS: 12053-18-8)?
The market for oxocopper (CAS: 12053-18-8) is primarily driven by its use in cat...
What is the market or research trend for 5-{[(2-Methyl-2-propanyl)oxy]carbonyl}-5-azaspiro[2.4]heptane-7-carboxylic acid?
The market for 5-{[(2-Methyl-2-propanyl)oxy]carbonyl}-5-azaspiro[2.4]heptane-7-c...
What is 2-(1-Pyrrolidinyl)-4-pyridinamine (CAS: 35981-63-6)?
2-(1-Pyrrolidinyl)-4-pyridinamine is a chemical compound with the CAS number 359...
What are the physical and chemical properties of 2-(3-Pyridinyl)-1-azabicyclo[2.2.2]octane (CAS: 91556-75-1)?
2-(3-Pyridinyl)-1-azabicyclo[2.2.2]octane (CAS: 91556-75-1) is a crystalline sol...
How is (S)-Alpha-allyl-proline hydrochloride (CAS: 129704-91-2) typically synthesized?
(S)-Alpha-allyl-proline hydrochloride is usually synthesized via a Wittig reacti...
What is 3-Methyl-1,2-oxazole-5-carboxylic acid (CAS: 4857-42-5)?
3-Methyl-1,2-oxazole-5-carboxylic acid (CAS: 4857-42-5) is an organic compound w...
How is Lys-SMCC-DM1 (CAS: 1281816-04-3) typically synthesized?
Lys-SMCC-DM1 is synthesized via a multi-step process involving the coupling of S...
Source Journal
Analyst

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.










![1-{3-[4-Amino-3-(4-phenoxyphenyl)-1H-pyrazolo[3,4-d]pyrimidin-1-yl]-1-piperidinyl}-2,3-dihydroxy-1-propanone structure 1-{3-[4-Amino-3-(4-phenoxyphenyl)-1H-pyrazolo[3,4-d]pyrimidin-1-yl]-1-piperidinyl}-2,3-dihydroxy-1-propanone structure](https://static.chemtradehub.com/structs/122/1226872-27-0-e037.webp)



