Zheng-Yuan Hong, Shu-Lin Liu, Dai-Wen Pang
The evaluation of the fluorescence intensity of single quantum dots (QDs) using a confocal fluorescence microscope can provide an alternative approach for estimating the effects of environmental changes or surface modifications on the fluorescence intensity of single QDs. In the case of blinking QDs, irregular blinking would significantly influence the intensity evaluation results that are based on the analysis of one or a few single QDs. In this regard, statistical intensity evaluations based on a large number of single QDs would be helpful to estimate an approximate intensity value of single QDs with reduced effects of blinking on the evaluation results. Herein, we developed a convenient method to statistically evaluate the fluorescence intensity of a large number of single blinking QDs using Gaussian distribution. Based on the intensity analysis of thousands of single QDs, the fluorescence intensity of the single QDs evaluated using a confocal fluorescence microscope was approximately 4090 with little data fluctuation induced by blinking.
Sílvia Alujas-Burgos, Pau Bayón, Marta Figueredo
DOI: 10.1039/C8OB01443D
Farhad Karimi
DOI: 10.1039/B209553J
Max Franz, Timo Stalling, Henning Steinert, Jürgen Martens
DOI: 10.1039/C8OB01865K
M. Lurdes S. Cristiano, David J. P. Gago, Antonio M. d'A. Rocha Gonsalves, Robert A. W. Johnstone, Moya McCarron
DOI: 10.1039/B210887A
Joshua A. Homer, Jonathan Sperry
DOI: 10.1039/C8OB02142B
DOI: 10.1039/B208827B
Jili Li, Jinlong Zhang, Qiuping Zhang, Zhongjie Bai, Quanyi Zhao, Dian He, Zhen Wang, Yonglin Chen, Bin Liu
DOI: 10.1039/C8OB01921E
Muhammad Ashram, Shehadeh Mizyed, Paris E. Georghiou
DOI: 10.1039/B209046P
Matthew J. Gaunt, Helen F. Sneddon, Peter R. Hewitt, Paolo Orsini, David F. Hook, Steven V. Ley.
DOI: 10.1039/B208982C
Anthony D. Wright, Claudia Osterhage, Gabriele M. König
DOI: 10.1039/B208588G
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