A simple and sensitive detection of small molecule–protein interactions based on terminal protection-mediated exponential strand displacement amplification

Literature Information

Publication Date 2018-03-21
DOI 10.1039/C8AN00099A
Impact Factor 4.616
Authors

Chang Yeol Lee, Hyo Yong Kim, Soeun Kim, Ki Soo Park, Hyun Gyu Park


View Original

Abstract

We herein describe a simple and sensitive strategy to detect a small molecule–protein interaction based on terminal protection-mediated exponential strand displacement amplification (eSDA). In principle, the small molecule linked to a DNA probe protects the DNA probe against the exonuclease I-catalyzed degradation after its binding to the corresponding target protein. The protected DNA probe then serves as a template to promote eSDA. Consequently, a large number of duplexes are produced, which leads to a high fluorescence from a double-stranded DNA specific fluorescent dye, SYBR Green I. As a model system to prove this sensing strategy, the interaction between biotin and streptavidin (SA), which is known to be the strongest among the non-covalent biological interactions, was selected and its analytical performance was thoroughly investigated. As a result, SA was sensitively detected with the limit of detection of 16 pM. In addition, the practical applicability of this method was successfully demonstrated by reliably determining the SA in human serum.

Related Literature

Heparin remodels the microtubule-binding repeat R3 of Tau protein towards fibril-prone conformations

Xuewei Dong, Ruxi Qi, Qin Qiao, Xuhua Li, Fangying Li, Jiaqian Wan, Qingwen Zhang, Guanghong Wei

2021-08-20 Paper

DOI: 10.1039/D1CP02651H

Origin of asynchronicity in Diels–Alder reactions

Pascal Vermeeren, Trevor A. Hamlin

2021-09-03 Paper

DOI: 10.1039/D1CP02456F

Surface electronic states mediate concerted electron and proton transfer at metal nanoscale interfaces for catalytic hydride reduction of –NO2 to –NH2

Bing-Qian Shan, Jia-Feng Zhou, Meng Ding, Xiao-Dan Hu

2021-05-25 Communication

DOI: 10.1039/D1CP01792F

Single-molecule binding characterization of primosomal protein PriA involved in replication restart

Tzu-Yu Lee, Yi-Ching Li, Min-Guan Lin, Chwan-Deng Hsiao, Hung-Wen Li

2021-05-25 Paper

DOI: 10.1039/D1CP00638J

Prediction of the standard potentials for one-electron oxidation of N,N,N′,N′ tetrasubstituted p-phenylenediamines by calculation

Cecilie L. Andersen, Evanildo G. Lacerda, Jr, Jørn B. Christensen, Stephan P. A. Sauer, Ole Hammerich

2021-09-02 Paper

DOI: 10.1039/D1CP02315B

Computational discovery of PtS2/GaSe van der Waals heterostructure for solar energy applications

Rui Xiong, Rong Hu, Yinggan Zhang, Xuhui Yang, Peng Lin, Cuilian Wen, Baisheng Sa, Zhimei Sun

2021-08-12 Paper

DOI: 10.1039/D1CP02436A

Front cover

2021-06-16 Cover

DOI: 10.1039/D1CP90121D

Room-temperature diffusion of metal clusters on graphene

Mohammad Zarshenas, Victor Gervilla, Davide G. Sangiovanni, Kostas Sarakinos

2021-05-24 Paper

DOI: 10.1039/D1CP00522G

You might also like

Compound Q&A

What is the market or research trend for N-(4-Methoxybenzyl)-2-pyridinamine (CAS: 52818-63-0)?

N-(4-Methoxybenzyl)-2-pyridinamine (CAS: 52818-63-0) is increasingly being used ...

52818-63-0N-(4-Methoxybenzyl)-...
Compound Q&A

What precautions should be taken when handling Ethyl 4-(2-chlorophenyl)-1,3-thiazole-2-carboxylate (CAS: 1050507-06-6)?

When handling Ethyl 4-(2-chlorophenyl)-1,3-thiazole-2-carboxylate, appropriate p...

1050507-06-6Ethyl 4-(2-chlorophe...
Compound Q&A

What regulatory guidelines apply to diethyldiselane (CAS: 628-39-7)?

Diethyldiselane (CAS: 628-39-7) is classified under the Globally Harmonized Syst...

628-39-7Diethyldiselane
Compound Q&A

What is the market or research trend for oxocopper (CAS: 12053-18-8)?

The market for oxocopper (CAS: 12053-18-8) is primarily driven by its use in cat...

12053-18-8oxocopper; oxo-(oxoc...
Compound Q&A

What is the market or research trend for 5-{[(2-Methyl-2-propanyl)oxy]carbonyl}-5-azaspiro[2.4]heptane-7-carboxylic acid?

The market for 5-{[(2-Methyl-2-propanyl)oxy]carbonyl}-5-azaspiro[2.4]heptane-7-c...

1268519-54-55-{[(2-Methyl-2-prop...
Compound Q&A

What is 2-(1-Pyrrolidinyl)-4-pyridinamine (CAS: 35981-63-6)?

2-(1-Pyrrolidinyl)-4-pyridinamine is a chemical compound with the CAS number 359...

35981-63-62-(1-Pyrrolidinyl)-4...
Compound Q&A

What are the physical and chemical properties of 2-(3-Pyridinyl)-1-azabicyclo[2.2.2]octane (CAS: 91556-75-1)?

2-(3-Pyridinyl)-1-azabicyclo[2.2.2]octane (CAS: 91556-75-1) is a crystalline sol...

91556-75-12-(3-Pyridinyl)-1-az...
Compound Q&A

How is (S)-Alpha-allyl-proline hydrochloride (CAS: 129704-91-2) typically synthesized?

(S)-Alpha-allyl-proline hydrochloride is usually synthesized via a Wittig reacti...

129704-91-2(S)-Alpha-allyl-prol...
Compound Q&A

What is 3-Methyl-1,2-oxazole-5-carboxylic acid (CAS: 4857-42-5)?

3-Methyl-1,2-oxazole-5-carboxylic acid (CAS: 4857-42-5) is an organic compound w...

4857-42-53-Methyl-1,2-oxazole...
Compound Q&A

How is Lys-SMCC-DM1 (CAS: 1281816-04-3) typically synthesized?

Lys-SMCC-DM1 is synthesized via a multi-step process involving the coupling of S...

1281816-04-3Lys-SMCC-DM1

Source Journal

Analyst

Analyst
CiteScore: 7.8
Self-citation Rate: 5.6%
Articles per Year: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

Recommended Compounds

Recommended Suppliers

Disclaimer
This page provides academic journal information for reference and research purposes only. We are not affiliated with any journal publishers and do not handle publication submissions. For publication-related inquiries, please contact the respective journal publishers directly.
If you notice any inaccuracies in the information displayed, please contact us at support@chemtradehub.com. We will promptly review and address your concerns.