Chemical analysis of multicellular tumour spheroids

Literature Information

Publication Date 2015-04-23
DOI 10.1039/C5AN00524H
Impact Factor 4.616
Authors

L. E. Jamieson, D. J. Harrison, C. J. Campbell


View Original

Abstract

Conventional two dimensional (2D) monolayer cell culture has been considered the ‘gold standard’ technique for in vitro cellular experiments. However, the need for a model that better mimics the three dimensional (3D) architecture of tissue in vivo has led to the development of Multicellular Tumour Spheroids (MTS) as a 3D tissue culture model. To some extent MTS mimic the environment of in vivo tumours where, for example, oxygen and nutrient gradients develop, protein expression changes and cells form a spherical structure with regions of proliferation, senescence and necrosis. This review focuses on the development of techniques for chemical analysis of MTS as a tool for understanding in vivo tumours and a platform for more effective drug and therapy discovery. While traditional monolayer techniques can be translated to 3D models, these often fail to provide the desired spatial resolution and z-penetration for live cell imaging. More recently developed techniques for overcoming these problems will be discussed with particular reference to advances in instrument technology for achieving the increased spatial resolution and imaging depth required.

Related Literature

Thermodiffusion of repulsive charged nanoparticles – the interplay between single-particle and thermoelectric contributions

M. Kouyaté, G. Demouchy, G. Mériguet, R. Aquino, E. Dubois, S. Nakamae, M. Roger, J. Depeyrot, R. Perzynski

2018-05-23 Paper

DOI: 10.1039/C8CP02558D

Correction: Surface induced smectic order in ionic liquids – an X-ray reflectivity study of [C22C1im]+[NTf2]−

Henning Weiss, Hailong Li, Oleg Konovalov, Sven Festersen, Uta Rütt

2018-09-12 Correction

DOI: 10.1039/C8CP91851A

Stability of metallo-porphyrin networks under oxygen reduction and evolution conditions in alkaline media

Diana Hötger, Markus Etzkorn, Benjamin Wurster, Jan Dreiser, Sebastian Stepanow, Doris Grumelli, Rico Gutzler

2019-01-11 Paper

DOI: 10.1039/C8CP07463A

White-light generation from all-solution-processed OLEDs using a benzothiazole–salophen derivative reactive to the ESIPT process

José Carlos Germino, Jônatas Faleiro Berbigier, Cristina Aparecida Barboza, Marcelo Meira Faleiros, Deborah de Alencar Simoni, Miguel Tayar Galante, Matheus Serra de Holanda, Fabiano Severo Rodembusch, Teresa Dib Zambon Atvars

2018-11-27 Paper

DOI: 10.1039/C8CP06485G

Li+-Induced fluorescent metallogel: a case of ESIPT-CHEF and ICT phenomenon

Manish Kumar Dixit, Mrigendra Dubey

2018-08-10 Paper

DOI: 10.1039/C8CP04579H

Model – free approach to quadrupole spin relaxation in solid 209Bi-aryl compounds

Danuta Kruk, Christian Goesweiner, Elzbieta Masiewicz, Evrim Umut, Carina Sampl, Hermann Scharfetter

2018-09-04 Paper

DOI: 10.1039/C8CP03848A

Atomistic dewetting mechanics of Wenzel and monostable Cassie–Baxter states

Senbo Xiao, Zhiliang Zhang, Jianying He

2018-08-24 Paper

DOI: 10.1039/C8CP03256D

The stabilization mechanism and size effect of nonpolar-to-polar crystallography facet tailored ZnO nano/micro rods via a top-down strategy

Xiangyang Duan, Chao Ma, Wentao Jin, Xiaoman Ma, Su-Huai Wei, Jinying Yu

2018-06-13 Paper

DOI: 10.1039/C8CP02494D

Strain and defect engineering on phase transition of monolayer black phosphorene

Yan Chen, Xiaoyang Shi, Mingjia Li, Yilun Liu

2018-06-06 Paper

DOI: 10.1039/C8CP01334A

You might also like

Compound Q&A

Is 2-(2-chloroacetamido)-3-phenylpropanoic acid (CAS: 7765-11-9) safe?

2-(2-Chloroacetamido)-3-phenylpropanoic acid (CAS: 7765-11-9) is generally consi...

7765-11-92-(2-chloroacetamido...
Compound Q&A

Is 2-(Benzyloxy)-5-bromobenzoic acid (CAS: 62176-31-2) safe?

2-(Benzyloxy)-5-bromobenzoic acid can be handled safely if appropriate precautio...

62176-31-22-(Benzyloxy)-5-brom...
Compound Q&A

What is (4-Methyl-1,2,5-oxadiazol-3-yl)methanamine hydrochloride (CAS: 1159825-48-5)?

(4-Methyl-1,2,5-oxadiazol-3-yl)methanamine hydrochloride is a chemical compound ...

1159825-48-5(4-Methyl-1,2,5-oxad...
Compound Q&A

What is 2-(5-Hexylthiophen-2-yl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane (CAS: 917985-54-7)?

2-(5-Hexylthiophen-2-yl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane (CAS: 917985-54...

917985-54-72-(5-Hexylthiophen-2...
Compound Q&A

Are there alternatives to 4-(8-Methyl-9H-1,3-dioxolo[4,5-h][2,3]benzodiazepin-5-yl)benzenamine (CAS: 102771-26-6) in synthesis?

While 4-(8-Methyl-9H-1,3-dioxolo[4,5-h][2,3]benzodiazepin-5-yl)benzenamine (CAS:...

102771-26-64-(8-Methyl-9H-1,3-d...
Compound Q&A

What is the market or research trend for tert-butyl 3-hydroxy-4,5,7,8-tetrahydro-2H-pyrazolo[3,4-d]azepine-6-carboxylate (CAS: 851376-80-2)?

The market for tert-butyl 3-hydroxy-4,5,7,8-tetrahydro-2H-pyrazolo[3,4-d]azepine...

851376-80-2tert-butyl 3-hydroxy...
Compound Q&A

How should waste containing 3,5-Diamino-1H-pyrazole-4-carbonitrile (CAS: 6844-58-2) be handled?

Waste containing 3,5-Diamino-1H-pyrazole-4-carbonitrile (CAS: 6844-58-2) should ...

6844-58-23,5-Diamino-1H-pyraz...
Compound Q&A

How is (6-Fluoro-3-pyridinyl)boronic acid (CAS: 351019-18-6) typically synthesized?

(6-Fluoro-3-pyridinyl)boronic acid can be synthesized through the reaction of 6-...

351019-18-6(6-Fluoro-3-pyridiny...
Compound Q&A

What industries use Dibenzyl carbonimidoylbiscarbamate (CAS: 10065-79-9)?

Dibenzyl carbonimidoylbiscarbamate (CAS: 10065-79-9) finds applications in vario...

10065-79-9Dibenzyl carbonimido...
Compound Q&A

What is the market or research trend for (beta,beta,2,3,4,5,6-~2~H_7_)Phenylalanine (CAS: 74228-83-4)?

The market for (beta,beta,2,3,4,5,6-~2~H_7_)Phenylalanine (CAS: 74228-83-4) is g...

74228-83-4(beta,beta,2,3,4,5,6...

Source Journal

Analyst

Analyst
CiteScore: 7.8
Self-citation Rate: 5.6%
Articles per Year: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

Recommended Compounds

Recommended Suppliers

Disclaimer
This page provides academic journal information for reference and research purposes only. We are not affiliated with any journal publishers and do not handle publication submissions. For publication-related inquiries, please contact the respective journal publishers directly.
If you notice any inaccuracies in the information displayed, please contact us at support@chemtradehub.com. We will promptly review and address your concerns.