Bare magnetic nanoparticles as fluorescence quenchers for detection of thrombin

Literature Information

Publication Date 2015-04-09
DOI 10.1039/C5AN00519A
Impact Factor 4.616
Authors

Liangrong Yang, Xiangfeng Liang


View Original

Abstract

Rapid and sensitive detection of thrombin has very important significance in clinical diagnosis. In this work, bare magnetic iron oxide nanoparticles (magnetic nanoparticles) without any modification were used as fluorescence quenchers. In the absence of thrombin, a fluorescent dye (CY3) labeled thrombin aptamer (named CY3-aptamer) was adsorbed on the surface of magnetic nanoparticles through interaction between a phosphate backbone of the CY3-aptamer and hydroxyl groups on the bare magnetic nanoparticles in binding solution, leading to fluorescence quenching. Once thrombin was introduced, the CY3-aptamer formed a G-quartet structure and combined with thrombin, which resulted in the CY3-aptamer being separated from the magnetic nanoparticles and restoration of fluorescence. This proposed assay took advantage of binding affinity between the CY3-aptamer and thrombin for specificity, and bare magnetic nanoparticles for fluorescence quenching. The fluorescence signal had a good linear relationship with thrombin concentration in the range of 1–60 nM, and the limit of detection for thrombin was estimated as low as 0.5 nM. Furthermore, this method could be applied for other target detection using the corresponding fluorescence labeled aptamer.

Related Literature

Shape transition of water-in-CO2 reverse micelles controlled by the surfactant midpiece

Muhan Wang, Junfeng Wang, Timing Fang, Youguo Yan, Zhiyuan Wang, Jun Zhang

2018-05-19 Paper

DOI: 10.1039/C8CP01844H

Contents list

Front/Back Matter

DOI: 10.1039/C8CP91781G

Tailoring the hexagonal boron nitride nanomesh on Rh(111) with gold

G. Vári, J. Kiss, A. Berkó

2018-05-08 Paper

DOI: 10.1039/C8CP00790J

Lithium doped tubular structure in LiB20 and LiB20−: a viable global minimum

Wei-yan Liang, Anita Das, Xue Dong, Zhong-hua Cui

2018-05-23 Paper

DOI: 10.1039/C8CP01376D

Ultrafast excited-state dynamics of 2,5-dimethylpyrrole

Zhichao Chen, Zhigang He, Kaijun Yuan, Dongxu Dai, Xueming Yang, Guorong Wu

2018-03-30 Paper

DOI: 10.1039/C8CP00883C

Understanding the interactions of imidazolium-based ionic liquids with cell membrane models

Carlos M. N. Mendonça, Debora T. Balogh, Simone C. Barbosa, Tânia E. Sintra, Sónia P. M. Ventura, Pedro Morgado, Eduardo J. M. Filipe, João A. P. Coutinho, Osvaldo N. Oliveira, Jr., Ana Barros-Timmons

2018-11-09 Paper

DOI: 10.1039/C8CP05035J

Electrocatalytic studies on imidazolium based ionic liquids: defining experimental conditions

Miguel A. Montiel, José Solla-Gullón, Vicente Montiel, Carlos M. Sánchez-Sánchez

2018-06-22 Paper

DOI: 10.1039/C8CP02662A

You might also like

Compound Q&A

Is 2-(2-chloroacetamido)-3-phenylpropanoic acid (CAS: 7765-11-9) safe?

2-(2-Chloroacetamido)-3-phenylpropanoic acid (CAS: 7765-11-9) is generally consi...

7765-11-92-(2-chloroacetamido...
Compound Q&A

Is 2-(Benzyloxy)-5-bromobenzoic acid (CAS: 62176-31-2) safe?

2-(Benzyloxy)-5-bromobenzoic acid can be handled safely if appropriate precautio...

62176-31-22-(Benzyloxy)-5-brom...
Compound Q&A

What is (4-Methyl-1,2,5-oxadiazol-3-yl)methanamine hydrochloride (CAS: 1159825-48-5)?

(4-Methyl-1,2,5-oxadiazol-3-yl)methanamine hydrochloride is a chemical compound ...

1159825-48-5(4-Methyl-1,2,5-oxad...
Compound Q&A

What is 2-(5-Hexylthiophen-2-yl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane (CAS: 917985-54-7)?

2-(5-Hexylthiophen-2-yl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane (CAS: 917985-54...

917985-54-72-(5-Hexylthiophen-2...
Compound Q&A

Are there alternatives to 4-(8-Methyl-9H-1,3-dioxolo[4,5-h][2,3]benzodiazepin-5-yl)benzenamine (CAS: 102771-26-6) in synthesis?

While 4-(8-Methyl-9H-1,3-dioxolo[4,5-h][2,3]benzodiazepin-5-yl)benzenamine (CAS:...

102771-26-64-(8-Methyl-9H-1,3-d...
Compound Q&A

What is the market or research trend for tert-butyl 3-hydroxy-4,5,7,8-tetrahydro-2H-pyrazolo[3,4-d]azepine-6-carboxylate (CAS: 851376-80-2)?

The market for tert-butyl 3-hydroxy-4,5,7,8-tetrahydro-2H-pyrazolo[3,4-d]azepine...

851376-80-2tert-butyl 3-hydroxy...
Compound Q&A

How should waste containing 3,5-Diamino-1H-pyrazole-4-carbonitrile (CAS: 6844-58-2) be handled?

Waste containing 3,5-Diamino-1H-pyrazole-4-carbonitrile (CAS: 6844-58-2) should ...

6844-58-23,5-Diamino-1H-pyraz...
Compound Q&A

How is (6-Fluoro-3-pyridinyl)boronic acid (CAS: 351019-18-6) typically synthesized?

(6-Fluoro-3-pyridinyl)boronic acid can be synthesized through the reaction of 6-...

351019-18-6(6-Fluoro-3-pyridiny...
Compound Q&A

What industries use Dibenzyl carbonimidoylbiscarbamate (CAS: 10065-79-9)?

Dibenzyl carbonimidoylbiscarbamate (CAS: 10065-79-9) finds applications in vario...

10065-79-9Dibenzyl carbonimido...
Compound Q&A

What is the market or research trend for (beta,beta,2,3,4,5,6-~2~H_7_)Phenylalanine (CAS: 74228-83-4)?

The market for (beta,beta,2,3,4,5,6-~2~H_7_)Phenylalanine (CAS: 74228-83-4) is g...

74228-83-4(beta,beta,2,3,4,5,6...

Source Journal

Analyst

Analyst
CiteScore: 7.8
Self-citation Rate: 5.6%
Articles per Year: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

Recommended Compounds

Recommended Suppliers

Disclaimer
This page provides academic journal information for reference and research purposes only. We are not affiliated with any journal publishers and do not handle publication submissions. For publication-related inquiries, please contact the respective journal publishers directly.
If you notice any inaccuracies in the information displayed, please contact us at support@chemtradehub.com. We will promptly review and address your concerns.