Artificial repressors for controlling gene expression in bacteria‡

Literature Information

Publication Date 2012-11-19
DOI 10.1039/C2CC37107C
Impact Factor 6.222
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Abstract

Transcriptional repression is a common approach to control gene expression in synthetic biology applications. Here, an engineered DNA binding protein based upon a transcription activator-like effector (TALE) scaffold was shown to outperform LacI in blocking transcription from a promoter and to repress expression of a downstream gene in an operon.

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