Using electrical and optical tweezers to facilitate studies of molecular motors

Literature Information

Publication Date 2009-04-16
DOI 10.1039/B821861G
Impact Factor 3.676
Authors

Mark E. Arsenault, Yujie Sun, Haim H. Bau, Yale E. Goldman


View Original

Abstract

Dielectrophoresis was used to stretch and suspend actin filaments across a trench etched between two electrodes patterned on a glass slide. Optical tweezers were used to bring a motor protein-coated bead into close proximity to a pre-selected, suspended actin filament, facilitating the attachment of the myosin-coated bead to the filament. The clearance beneath the filament allowed the bead to move freely along and around its filamentous track, unhindered by solid surfaces. Using defocused images, the three-dimensional position of the bead was tracked as a function of time to obtain its trajectory. Experiments were carried out with myosin V and myosin X. Both motor proteins followed left-handed helical paths with the myosin X motor exhibiting a shorter pitch than the myosin V. The combined use of electrostatic and optical tweezers facilitates the preparation of motility assays with suspended tracks. Variants of this technique will enable higher complexity experiments in vitro to better understand the behavior of motors in cells.

Related Literature

C–H bond chlorination using nickel(ii) complexes of tetradentate amido-quinoline ligands

Sanjay Adhikari, Aniruddha Sarkar, Basab Bijayi Dhar

2022-02-28 Communication

DOI: 10.1039/D2CC00639A

Nanocomposites of bacterial cellulose nanofibers and chitin nanocrystals: fabrication, characterization and bactericidal activity

Núria Butchosa, Christian Brown, Lars A. Berglund, Vincent Bulone

2013-09-17 Paper

DOI: 10.1039/C3GC41700J

Dispersion of PM and VOC pollutants from open burning of municipal solid wastes on host communities: emission inventory estimation and dispersion modelling study

Adewemimo Oluwakunmi Popoola, Lukuman Adekilekun Jimoda, Olusesan Abel Olu-Arotiowa, Oyetola Ogunkunle, Opeyeolu Timothy Laseinde, Sunday Adekunle Adebanjo, Wuraola Abake Raji

2023-06-02 Paper

DOI: 10.1039/D3EA00041A

Studying manganese carbonyl photochemistry in a permanently porous metal–organic framework

Michael T. Huxley, Lingjun Wu, Jack Hart, James O'Shea, Christian J. Doonan, Christopher J. Sumby

2023-08-15 Edge Article

DOI: 10.1039/D3SC03553K

Engineering the outcome of cofermentation processes by altering the feedstock sugar-to-protein ratio

R. Bevilacqua, M. Mauricio-Iglesias, S. Balboa, J. M. Lema, M. Carballa

2022-05-26 Paper

DOI: 10.1039/D2EW00144F

Palladium catalysed enantioselective phosphination reactions using secondary phosphine-boranes and aryl iodide

Stéphanie Pican, Annie-Claude Gaumont

2005-03-15 Communication

DOI: 10.1039/B501078K

Cocatalyst engineering to weaken the charge screening effect over Au–Bi4Ti3O12 for piezocatalytic pure water splitting

Rui Lei, Xianzhi Fu, Naxin Chen, Yifeng Chen, Wenhui Feng, Ping Liu

2022-11-08 Paper

DOI: 10.1039/D2CY01422J

Synthesis of K[B3H7NH2BH2NH2B3H7] for a K-ion solid-state electrolyte

Xi-Meng Chen, Si-Han Jia, Jia-Xin Kang, Yichun Zhang, Yubin Ma, Yiming Ma, Xin Jiang, Xing-Chao Yu, Pengtao Qiu

2022-03-04 Communication

DOI: 10.1039/D2CC00408A

You might also like

Compound Q&A

What industries use 4-(4-tert-Butylphenyl)-1H-pyrazol-3-amine (CAS: 1015845-73-4)?

4-(4-tert-Butylphenyl)-1H-pyrazol-3-amine finds applications in various industri...

1015845-73-44-(4-tert-Butylpheny...
Compound Q&A

What industries use H3TATAB (CAS: 63557-10-8)?

H3TATAB is used in the pharmaceutical industry for the synthesis of certain orga...

63557-10-8H3TATAB
Compound Q&A

What are the main uses of 1-Ethyl-3-fluorobenzene (CAS: 696-39-9)?

1-Ethyl-3-fluorobenzene (CAS: 696-39-9) is primarily used as a precursor in the ...

696-39-91-Ethyl-3-fluorobenz...
Compound Q&A

What are the main uses of 1-(tert-Butoxycarbonyl)-4-(4-methoxyphenyl)pyrrolidine-3-carboxylic acid (CAS: 851484-94-1)?

1-(tert-Butoxycarbonyl)-4-(4-methoxyphenyl)pyrrolidine-3-carboxylic acid is prim...

851484-94-11-(tert-Butoxycarbon...
Compound Q&A

What are the physical and chemical properties of 1-Cyclobutyl-4-piperidinone (CAS: 359880-05-0)?

1-Cyclobutyl-4-piperidinone (CAS: 359880-05-0) is a colorless or white crystalli...

359880-05-01-Cyclobutyl-4-piper...
Compound Q&A

What is Pyridine-2,6-dicarboxylic acid mono-tert-butyl ester (CAS: 575433-76-0)?

Pyridine-2,6-dicarboxylic acid mono-tert-butyl ester (CAS: 575433-76-0) is a che...

575433-76-0Pyridine-2,6-dicarbo...
Compound Q&A

What is the market or research trend for 2,3-Difluorophenylalanine (CAS: 236754-62-4)?

The market for 2,3-Difluorophenylalanine (CAS: 236754-62-4) is growing with incr...

236754-62-42,3-Difluorophenylal...
Compound Q&A

How is (2-Hydroxy-1-naphthyl)boronic acid (CAS: 898257-48-2) typically synthesized?

(2-Hydroxy-1-naphthyl)boronic acid can be synthesized through the reduction of 2...

898257-48-2(2-Hydroxy-1-naphthy...
1315351-28-0tert-Butyl (5-bromo-...
Compound Q&A

Are there alternatives to 5,7-Dihydroxy-4-oxo-2-(3,4,5-trihydroxyphenyl)-4H-chromen-3-yl beta-D-glucopyranoside (CAS: 19833-12-6) in synthesis?

While 5,7-Dihydroxy-4-oxo-2-(3,4,5-trihydroxyphenyl)-4H-chromen-3-yl beta-D-gluc...

19833-12-65,7-Dihydroxy-4-oxo-...

Source Journal

Physical Chemistry Chemical Physics

Physical Chemistry Chemical Physics
CiteScore: 5.5
Self-citation Rate: 10.3%
Articles per Year: 3036

Physical Chemistry Chemical Physics (PCCP) is an international journal co-owned by 19 physical chemistry and physics societies from around the world. This journal publishes original, cutting-edge research in physical chemistry, chemical physics and biophysical chemistry. To be suitable for publication in PCCP, articles must include significant innovation and/or insight into physical chemistry; this is the most important criterion that reviewers and Editors will judge against when evaluating submissions. The journal has a broad scope and welcomes contributions spanning experiment, theory, computation and data science. Topical coverage includes spectroscopy, dynamics, kinetics, statistical mechanics, thermodynamics, electrochemistry, catalysis, surface science, quantum mechanics, quantum computing and machine learning. Interdisciplinary research areas such as polymers and soft matter, materials, nanoscience, energy, surfaces/interfaces, and biophysical chemistry are welcomed if they demonstrate significant innovation and/or insight into physical chemistry. Joined experimental/theoretical studies are particularly appreciated when complementary and based on up-to-date approaches.

Recommended Compounds

Recommended Suppliers

Disclaimer
This page provides academic journal information for reference and research purposes only. We are not affiliated with any journal publishers and do not handle publication submissions. For publication-related inquiries, please contact the respective journal publishers directly.
If you notice any inaccuracies in the information displayed, please contact us at support@chemtradehub.com. We will promptly review and address your concerns.