Effect of sample volume on the limit of detection in flow injection hydride generation electrothermal atomic absorption spectrometry
Literature Information
The analytical performance of methods for the determination of hydride forming elements has been improved recently by the development of procedures in which the hydride is trapped on the interior surface of a graphite furnace atomizer. The signal for a given concentration increases with increase in sample volume and it is often implied that a decrease in the limit of detection may also be achieved by increasing the sample volume. To evaluate this claim, a simple equation was derived which predicts the relationship between detection limit and sample volume when all the contributions to the blank are proportional to sample volume. A time-based approach to the variation of sample volume was developed to ensure that the analyte introduced from reagent contamination was, in fact, proportional to sample volume. Detection limits were measured for a series of sample volumes between 156 and 1560 µl. As the sample volume was increased, the detection limit improved significantly from 0.3 to around 0.05 µg l–1 up to a volume of about 500 µl. Between 500 and 1000 µl, a further improvement, to around 0.02 µg l–1, was obtained, but for volumes larger than 1000 µl no further significant improvement was obtained. Good agreement between the predicted and experimentally determined variations in detection limit with sample volume was obtained and thus the underlying inverse proportionality of the relationship between detection limit and sample volume was confirmed. This rectangular hyperbolic relationship has practical consequences for the extent to which detection limits can be improved by increasing the sample volume, even when the blank is very low or zero.
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Journal of Analytical Atomic Spectrometry

The Journal of Analytical Atomic Spectrometry (JAAS) is the central journal for publishing innovative research on fundamentals, instrumentation, and methods in the determination, speciation and isotopic analysis of (trace) elements within all fields of application. This includes, but is not restricted to, the most recent progress, developments and achievements in all forms of atomic and elemental detection, isotope ratio determination, molecular analysis, plasma-based analysis and X-ray techniques. The journal welcomes full papers, communications, technical notes, critical and tutorial review articles, editorials, and comments, in addition to the Atomic Spectrometry Updates (ASU) literature reviews that are prepared by an expert panel. Submissions are welcome in the following areas, but note this list reflects the current scope and authors are strongly encouraged to contact the Editorial team if they believe that their work offers potentially new and emerging research relevant to the journal remit: Fundamental studies in the following. New and existing sources for atomic emission, absorption, fluorescence and mass spectrometry and those that provide both atomic and molecular information Sample introduction techniques for solids, liquids, gases Improvements in sensitivity, selectivity, precision, accuracy and/or robustness Isotope ratio measurements, including techniques for improving precision and mass bias correction Single channel and multichannel simultaneous detection systems Chemometrics, statistics, calibration techniques and internal standardisation Theoretical and numerical modelling of fundamental processes related to all of the above methodologies Novel or improved methodologies in areas of application including, but not limited to the following. Biosciences, including elemental, speciation and isotopic analysis in biological systems, immunoassays based on metal-labeled antibodies, bio-imaging, and nanoparticle toxicology Geochemistry Environmental science Materials science, including engineered nanoparticles and quantum dots Metrology, including reference materials Forensic analysis Food and agricultural sciences Energy Archaeometry Molecular analysis. Molecular sources for elemental and isotopic analysis Atomic sources for molecular analysis Atomic and molecular techniques simultaneously used for complementary chemical information All contributions are judged on originality and quality of scientific content, and appropriateness of length to content of new science.










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