HEPES | CAS No. 7365-45-9

HEPES

Basic Information

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CAS Number

7365-45-9

Molecular Formula

C8H18N2O4S

Molecular Weight

238.31 g/mol

Chemical Classification

Basic Chemical Raw Materials Organic Chemicals Carboxylic Acids

Basic Chemical Raw Materials Organic Chemicals Heterocyclic Compounds

Pharmaceutical Chemicals Pharmaceutical Intermediates Heterocyclic Intermediates

Basic Physical Properties

Melting Point

234-238 ºC

Density

1.07 g/mL at 20 °C

Solubility

H2O: 1 M at 20 °C, clear, colorless

Refractive Index

n20/D 1.339

SMILES

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C1CN(CCN1CCO)CCS(=O)(=O)O

InChI

InChI=1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChIKey

JKMHFZQWWAIEOD-UHFFFAOYSA-N

LogP

-1.5158999999999976

pKa

7.5(at 25℃)

Topological Polar Surface Area

81.08 Å²

Hydrogen Bond Donor/Acceptor

2 / 6

Complexity

267

Safety Information

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GHS Hazard Pictograms

H315

H319

Hazard Statement

H315 Causes skin irritation
Skin corrosion/irritation

H319 Causes serious eye irritation
Serious eye damage/eye irritation

Stability

Stable. Combustible. Incompatible with strong oxidizing agents. Protect from moisture.

Synonyms & References

English

F20297
HEPES-buffered saline, pH 7.0 (2X for transfection)
HEPES, Pharmaceutical Secondary Standard; Certified Reference Material
Epitope ID:190604
HEPES
N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid
1d1p
4-(2-hydroxyethyl)-1-piperazine ethanesulphonic acid
CHEMBL1232545
2-[4-(2-hydroxyethyl)-1-piperazinyl] ethanesulfonic acid
15(R)-hydroperoxy-EPE
4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid
203805-87-2
4-(2-hydroxyethyl)-1-piperazine ethanesulphonate
2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethane-1-sulfonic acid
HEPES, Vetec(TM) reagent grade, 99.5%
FT-0767998
N-2-Hydroxyethylpiperazine-N'-ethanesulfonic acid
NSC 166663
DTXSID5040382
7365-45-9
UNII-RWW266YE9I
N-2-Hydroxyethylpiperazino-N'-2-ethanesulfonic acid
BS-17486
4-(2-Hydroxyethyl)-1-piperazineethane sulfonate
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid
2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulfonic acid
N-2-HYDROXYETHYLPIPERAZINE N'-2'-ETHANESULPHONIC ACID
CHEBI:42334
2-[4-(2-hydroxyethyl)-1-piperazinyl]ethane sulfonic acid

MDL Number

MFCD00006158

Customs Code

29335995

FAQ

What is HEPES (CAS: 7365-45-9)?

HEPES, or N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid (CAS: 7365-45-9), is a zwitterionic buffering agent used to maintain stable pH levels in aqueous solutions. It has a chemical formula of C8H18N2O4S, is highly soluble in water, and exhibits excellent pH buffering capacity between pH 6.8 and 8.2. HEPES is commonly used in biological and biochemical applications due to its low toxicity and compatibility with cellular systems.

What are the main uses of HEPES (CAS: 7365-45-9)?

HEPES (CAS: 7365-45-9) is widely utilized in pharmaceuticals, research, and biotechnology. It serves as a buffering agent in cell culture media, enzyme assays, and pharmaceutical formulations. In research, HEPES stabilizes pH in buffer solutions for experiments involving proteins, nucleic acids, and cells. Industrially, it is used in detergents and as a preservative. Its applications also extend to cosmetics and pharmaceutical excipients for pH control.

Is HEPES (CAS: 7365-45-9) safe?

HEPES (CAS: 7365-45-9) is generally considered safe with low toxicity when handled properly. It is non-irritating and non-toxic to humans and aquatic life, making it suitable for biological applications. However, standard safety precautions should be followed, such as wearing gloves and ensuring adequate ventilation. It meets regulatory safety standards and is approved for use in pharmaceutical and research contexts.

How should HEPES (CAS: 7365-45-9) be stored?

HEPES (CAS: 7365-45-9) should be stored in a cool, dry place (15-30°C), away from light, and in airtight containers to prevent moisture absorption. It remains stable under normal storage conditions but should be protected from contamination. Storage in dark, sealed containers ensures long-term shelf life and maintains its buffering properties. Avoid exposure to extreme temperatures or humidity to preserve chemical integrity.