HEPES(CAS: 7365-45-9)

HEPES, or N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid (CAS: 7365-45-9), is a zwitterionic buffering agent used to maintain stable pH levels in aqueous solutions. It has a chemical formula of C8H18N2O4S, is highly soluble in water, and exhibits excellent pH buffering capacity between pH 6.8 and 8.2. HEPES is commonly used in biological and biochemical applications due to its low toxicity and compatibility with cellular systems.

HEPES (CAS: 7365-45-9) is widely utilized in pharmaceuticals, research, and biotechnology. It serves as a buffering agent in cell culture media, enzyme assays, and pharmaceutical formulations. In research, HEPES stabilizes pH in buffer solutions for experiments involving proteins, nucleic acids, and cells. Industrially, it is used in detergents and as a preservative. Its applications also extend to cosmetics and pharmaceutical excipients for pH control.

HEPES (CAS: 7365-45-9) is generally considered safe with low toxicity when handled properly. It is non-irritating and non-toxic to humans and aquatic life, making it suitable for biological applications. However, standard safety precautions should be followed, such as wearing gloves and ensuring adequate ventilation. It meets regulatory safety standards and is approved for use in pharmaceutical and research contexts.

HEPES (CAS: 7365-45-9) should be stored in a cool, dry place (15-30°C), away from light, and in airtight containers to prevent moisture absorption. It remains stable under normal storage conditions but should be protected from contamination. Storage in dark, sealed containers ensures long-term shelf life and maintains its buffering properties. Avoid exposure to extreme temperatures or humidity to preserve chemical integrity.