TMB dihydrochloride

Basic Information

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CAS Number

64285-73-0

Molecular Formula

C16H22Cl2N2

Molecular Weight

313.3 g/mol

Chemical Classification

Basic Chemical Raw Materials Organic Chemicals Amines

Agricultural Chemicals Pesticides Herbicides

Basic Physical Properties

Melting Point

≥300 °C

Boiling Point

368.6 °C at 760 mmHg

Density

0.8920

Flash Point

54 °C

SMILES

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CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C.Cl.Cl

InChI

InChI=1S/C16H20N2.2ClH/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;/h5-8H,17-18H2,1-4H3;2*1H

InChIKey

NYNRGZULARUZCC-UHFFFAOYSA-N

LogP

4.595280000000004

Topological Polar Surface Area

52.04 Å²

Hydrogen Bond Donor/Acceptor

4 / 2

Complexity

226

Safety Information

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Hazard Statement

H315 Causes skin irritation
Skin corrosion/irritation

H319 Causes serious eye irritation
Serious eye damage/eye irritation

H335 May cause respiratory irritation
Specific target organ toxicity, single exposure; Respiratory tract irritation

Sensitiveness

Moisture & Light Sensitive

Synonyms & References

English

3,3',5,5'-Tetramethylbenzidine dihydrochloride
TMB dihydrochloride
EINECS 264-769-6
BCP31645
AKOS015966729
FT-0614008
A834702
(1,1'-Biphenyl)-4,4'-diamine, 3,3',5,5'-tetramethyl-, dihydrochloride
3,3',5,5'-Tetramethylbenzidine, dihydrochloride hydrate
3,3',5,5'-Tetramethyl(1,1'-biphenyl)-4,4'-diamine dihydrochloride
O10900
SCHEMBL472235
C16H22Cl2N2
207738-08-7
3,3',5,5'-Tetramethylbenzidine dihydrochloride anhydrous
64285-73-0
4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline;dihydrochloride
DS-13669
AC-24636
T-2150
4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline dihydrochloride
3,3',5,5'-Tetramethylbenzidine DiHCl
s6822
3,3',5,5'-Tetramethylbenzidine dihydrochloride, tablet, 1 mg substrate per tablet
BM blue dihydrochloride hydrate;Sure Blue TMB dihydrochloride hydrate
3,3,5,5-TETRAMETHYLBENZIDINEDIHYDROCHLORIDE
FT-0660577
(1,1'-Biphenyl)-4,4'-diamine, 3,3',5,5'-tetramethyl-, hydrochloride (1:2)
4,4'-Diamino-3,3',5,5'-tetramethylbiphenyl dihydrochloride
TMB (dihydrochloride hydrate)
MFCD00150104

MDL Number

MFCD00012961

Customs Code

29215990

FAQ

What is TMB dihydrochloride (CAS: 64285-73-0)?

TMB dihydrochloride, with the CAS number 64285-73-0, is a chemical compound commonly used as a chromogenic substrate in biochemical assays. It has the molecular formula C10H13N3O2·2HCl and is known for its stability in aqueous solutions. This compound is typically yellow in color and has a molecular weight of approximately 273.7 g/mol. It is widely recognized for its role in enzyme-linked immunosorbent assays (ELISA) and other diagnostic applications due to its strong absorption properties in the visible spectrum.

What are the main uses of TMB dihydrochloride (CAS: 64285-73-0)?

TMB dihydrochloride is primarily used in enzyme-linked immunosorbent assays (ELISA) as a chromogenic substrate for horseradish peroxidase (HRP). It is also applied in immunohistochemistry, immunoassays, and other biochemical research fields. Its sensitivity and ease of use make it a popular choice for detecting antigens and antibodies. Additionally, it finds applications in environmental testing and industrial quality control due to its reactivity and color development properties.

Is TMB dihydrochloride (CAS: 64285-73-0) safe?

TMB dihydrochloride is generally considered safe when handled properly, but it should be treated with standard laboratory precautions. It may cause mild irritation to the skin and eyes, and inhalation of its vapors should be avoided. Safety data sheets (SDS) recommend using gloves, goggles, and a fume hood during handling. It is not classified as highly toxic, but proper disposal and adherence to safety guidelines are essential to ensure safe use in research and industrial settings.

How should TMB dihydrochloride (CAS: 64285-73-0) be stored?

TMB dihydrochloride should be stored in a cool, dry place away from direct light. It is typically kept in airtight containers at a temperature range of 2-8°C to maintain stability and prevent degradation. Proper storage conditions are crucial to preserve its chemical integrity and ensure consistent performance in assays. Avoid exposure to moisture and ensure the container is sealed tightly to maintain quality over time.