Hoechst 33258 trihydrochloride

Basic Information

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CAS Number

23491-45-4

Molecular Formula

C25H27Cl3N6O

Molecular Weight

460.97 g/mol

Chemical Classification

Basic Chemical Raw Materials Organic Chemicals Heterocyclic Compounds

Pharmaceutical Chemicals Pharmaceutical Intermediates Heterocyclic Intermediates

Agricultural Chemicals Pesticides Herbicides

Laboratory Chemicals Research Chemicals Biochemical Reagents

Basic Physical Properties

Melting Point

280°C

Boiling Point

643.1 °C at 760 mmHg

Flash Point

342.7 °C

Solubility

H2O: 10 mg/mL

Refractive Index

1.6300 (estimate)

SMILES

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CN1CCN(CC1)C2=CC3=C(C=C2)N=C(N3)C4=CC5=C(C=C4)N=C(N5)C6=CC=C(C=C6)O.Cl.Cl.Cl

InChI

InChI=1S/C25H24N6O.ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16;/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29);1H

InChIKey

UDJDXLXTHZQAAC-UHFFFAOYSA-N

LogP

4.6524000000000045

Topological Polar Surface Area

84.07 Å²

Hydrogen Bond Donor/Acceptor

3 / 7

Complexity

634

Safety Information

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GHS Hazard Pictograms

H302

H315

H319

Hazard Statement

H302 Harmful if swallowed
Acute toxicity, oral

H315 Causes skin irritation
Skin corrosion/irritation

H319 Causes serious eye irritation
Serious eye damage/eye irritation

Synonyms & References

English

(2'-(4-Hydroxyphenyl)-5-(4-Methyl-1-Piperazinyl)-2,5'-Bi(1H-Benzimidazole) Trihydrochloride
4-(5-(4-methylpiperazin-1-yl)-1H,1'H-2,5'-bibenzo[d]imidazol-2'-yl)phenol trihydrochloride
2'-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole Trihydrochloride
Hoechst Dye 33258
2 inverted exclamation mark -(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5 inverted exclamation mark -bibenzimidazole Trihydrochloride
SY057920
BBIH
H1343
Pibenzimol hydrochloride
Bisbenzimide H 33258 Fluorochrome, Trihydrochloride - CAS 23491-45-4
Hoechst 33258 3HCl
HOE 33258
AS-75157
BCP19630
Phenol, 4-(6-(4-methyl-1-piperazinyl)(2,6'-bi-1H-benzimidazol)-2'-yl)-, hydrochloride (1:3)
AKOS030632783
2-(2-(4-HYDROXYPHENYL)-6-BENZIMIDAZOLYL)-6-(1-METHYL-4-PIPERZYL)
CS-7568
23491-45-4 (HCl)
4-[6-(4-Methyl-1-piperazinyl)[2,6'-bi-1H-benzimidazol]-2'-yl]phenol trihydrochloride
HB0786
HOECHST 33258
4-(5-(4-Methyl-1-piperazinyl)(2,5'-bi-1H-benzimidazol)-2'-yl)phenol trihydrochloride
HY-15558A
CHEMBL34922
bisBenzimide H 33258
C25H24N6O 3HCl
BISBENZIMIDE TRIHYDROCHLORIDE
Q27284034
H 33258
p-(5-(4-Methyl-1-piperazinyl)(2,5'-bi-1H-benzimidazol)-2'-yl)phenol trihydrochloride

MDL Number

MFCD00012679

FAQ

What regulatory guidelines apply to Hoechst 33258 trihydrochloride (CAS: 23491-45-4)?

Hoechst 33258 trihydrochloride (CAS: 23491-45-4) is subject to several regulatory guidelines. It falls under the scope of the REACH regulations in Europe, which require registration, evaluation, authorization, and restriction of chemicals. Additionally, it must comply with the Globally Harmonized System of Classification and Labelling of Chemicals (GHS) for hazardous product labeling. In the United States, the FDA and EPA may also have specific requirements for its handling and use, including safety data sheets and proper storage conditions.

Are there alternatives to Hoechst 33258 trihydrochloride (CAS: 23491-45-4) in synthesis?

While Hoechst 33258 trihydrochloride (CAS: 23491-45-4) is a well-established compound, alternatives such as other DNA stains like YOYO-1 and SYTOX Green are available. These alternatives may offer similar fluorescence properties but with different synthesis methods or less stringent regulatory requirements. It's important to evaluate the specific needs of your application to determine the most appropriate reagent.

What is the market or research trend for Hoechst 33258 trihydrochloride (CAS: 23491-45-4)?

Hoechst 33258 trihydrochloride (CAS: 23491-45-4) remains a popular choice in research due to its high affinity for DNA and its ability to stain nucleic acids. Market trends show a steady demand for its use in cell imaging and fluorescence microscopy. Recent research is focusing on its applications in live cell imaging and its potential use in drug discovery. However, ongoing developments in fluorescent probes are likely to introduce newer alternatives, potentially shifting market dynamics.

How should waste containing Hoechst 33258 trihydrochloride (CAS: 23491-45-4) be handled?

Waste containing Hoechst 33258 trihydrochloride (CAS: 23491-45-4) should be collected in appropriate containers and stored separately to prevent contamination. Upon collection, it should be neutralized to minimize environmental impact. For disposal, incineration is a common method, but professional waste disposal services should be utilized to ensure compliance with local regulations. Proper handling and disposal should also include precautions to protect the environment and human health.