Inkjet-printed micro-calibration standards for ultraquantitative Raman spectral cytometry
Literature Information
Publication Date
2019-05-16
DOI
10.1039/C9AN00500E
Impact Factor
4.616
Authors
Vernon LaLone, Maria V. Fawaz, Jomar Morales-Mercado, Márcio A. Mourão, Catherine S. Snyder, Sang Yeop Kim, Andrew P. Lieberman, Theodore J. Standiford, Krishnan Raghavendran, Kerby Shedden, Anna Schwendeman, Gus R. Rosania
View Original
Abstract
Herein we report the development of a cytometric analysis platform for measuring the contents of individual cells in absolute (picogram) scales; this study represents the first report of Raman-based quantitation of the absolute mass – or the total amount – of multiple endogenous biomolecules within single-cells. To enable ultraquantitative calibration, we engineered single-cell-sized micro-calibration standards of known composition by inkjet-printer deposition of biomolecular components in microarrays across the surface of silicon chips. We demonstrate clinical feasibility by characterizing the compositional phenotype of human skin fibroblast and porcine alveolar macrophage cell populations in the respective contexts of Niemann–Pick disease and drug-induced phospholipidosis: two types of lipid storage disorders. We envision this microanalytical platform as the foundation for many future biomedical applications, ranging from diagnostic assays to pathological analysis to advanced pharmaco/toxicokinetic research studies.
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Analyst
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Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.
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