Photoinduced dimerization of a photosensory DNA-binding protein EL222 and its LOV domain
Literature Information
Akira Takakado, Yusuke Nakasone, Masahide Terazima
EL222 is a blue light sensor protein consisting of a light-oxygen-voltage (LOV) domain (EL-LOV domain) at the N-terminus and a helix-turn-helix DNA-binding domain at the C-terminus. EL222 acts as a light dependent transcriptional factor. The photochemical reactions of EL222 and the light sensing properties of the LOV domain were investigated. Concentration dependent experiments revealed that the EL-LOV domain is in equilibrium between the dimer and the monomer in the dark state, and the main photoreaction is the dimerization reaction between a monomer in the ground state and that in the excited state. The equilibrium constant and the intrinsic rate constants of dimerization were determined. EL222 was found to also exhibit photoinduced dimerization even in the absence of target DNA, although the yield of the reaction was low (∼0.08 compared with that of the EL-LOV domain). This observation suggests that there are inhomogeneous conformations, open and closed types, of EL222 in solution.
Related Literature
Synthesis of ellipsoidal hematite/polymer/titania hybrid materials and the corresponding hollow ellipsoidal particles
Longyu Li, Dianbin Qin, Xinlin Yang, Guangyu Liu
DOI: 10.1039/B9PY00230H
Synthesis of thermoresponsive phenyl- and naphthyl-terminated poly(NIPAM) derivatives using RAFT and their complexation with cyclobis(paraquat-p-phenylene) derivatives in water
Graeme Cooke
DOI: 10.1039/C0PY00085J
Thermo-responsive cellulose-based architectures: tailoring LCST using poly(ethylene glycol) methacrylates‡
Christian Porsch, Susanne Hansson, Niklas Nordgren, Eva Malmström
DOI: 10.1039/C0PY00417K
Characterization of network structure in radiation-cured resins of polyfunctional acrylic ester and N-vinylpyrrolidone by MALDI-MS combined with supercritical methanolysis
Hideki Matsubara, Hiroyasu Kataoka, Hajime Ohtani
DOI: 10.1039/C0PY00026D
Conjugated polyelectrolyte as signal amplifier for fluorogenic probe based enzyme activity study
Ruoyu Zhan, Angela Jun Hiong Tan, Bin Liu
DOI: 10.1039/C0PY00265H
Free radical polymerization of alkyl methacrylates with N,N-dimethylanilinium p-toluenesulfonate at above ambient temperature: a quasi-living system
Atanu Kotal, Tapas K. Paira, Sanjib Banerjee, Chinmoy Bhattacharya, Tarun K. Mandal
DOI: 10.1039/C0PY00180E
Recent advances in entropy-driven ring-opening polymerizations
Satu Strandman, Julien E. Gautrot, X. X. Zhu
DOI: 10.1039/C0PY00328J
The role of responsive branched copolymer composition in controlling pH-triggered aggregation of “engineered” emulsion droplets: towards selective droplet assembly‡
Robert T. Woodward, Jonathan V. M. Weaver
DOI: 10.1039/C0PY00277A
Self-assembly and thermodynamic synthesis of rotaxanedendrimers and related structures
Ken Cham-Fai Leung, Kwun-Ngai Lau
DOI: 10.1039/B9PY00380K
You might also like
Is 6-(3-Fluorophenyl)picolinic acid (CAS: 887982-40-3) safe?
6-(3-Fluorophenyl)picolinic acid is generally considered safe for laboratory use...
What industries use (3R)-3-Pyrrolidinol (CAS: 2799-21-5)?
(3R)-3-Pyrrolidinol is used in the pharmaceutical industry as a precursor for dr...
What precautions should be taken when handling (4R,5R)-4,5-Diethoxycarbonyl-2,2-dimethyldioxolane (CAS: 59779-75-8)?
When handling (4R,5R)-4,5-Diethoxycarbonyl-2,2-dimethyldioxolane (CAS: 59779-75-...
How is 1-(6-Chloroimidazo[1,2-b]pyridazin-3-yl)ethanone (CAS: 90734-71-7) typically synthesized?
1-(6-Chloroimidazo[1,2-b]pyridazin-3-yl)ethanone is often synthesized via a mult...
What is the market or research trend for N-Ethyl-3,4-dimethylbenzylamine (CAS: 39180-83-1)?
The market for N-Ethyl-3,4-dimethylbenzylamine (CAS: 39180-83-1) remains steady,...
What is Tert-butyl 3-(pyrrolidin-1-yl)azetidine-1-carboxylate (CAS: 1019008-21-9)?
Tert-butyl 3-(pyrrolidin-1-yl)azetidine-1-carboxylate is a chemical compound wit...
What regulatory guidelines apply to 1-Bromo-3-chloro-2,4-dimethoxybenzene (CAS: 1228956-93-1)?
1-Bromo-3-chloro-2,4-dimethoxybenzene (CAS: 1228956-93-1) falls under the classi...
Is 8-Bromo-2-methyl-3,4-dihydroisoquinolin-1(2H)-one (CAS: 1368622-07-4) safe?
The safety of 8-Bromo-2-methyl-3,4-dihydroisoquinolin-1(2H)-one (CAS: 1368622-07...
Is Benzyl [(3S)-2,6-dioxo-3-piperidinyl]carbamate (CAS: 22785-43-9) safe?
Benzyl [(3S)-2,6-dioxo-3-piperidinyl]carbamate is generally safe when handled wi...
How should 1-{[4-(4,4,5,5-Tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl]sulfonyl}pyrrolidine (CAS: 928657-21-0) be stored?
1-{[4-(4,4,5,5-Tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl]sulfonyl}pyrrolidine s...
Source Journal
Physical Chemistry Chemical Physics

Physical Chemistry Chemical Physics (PCCP) is an international journal co-owned by 19 physical chemistry and physics societies from around the world. This journal publishes original, cutting-edge research in physical chemistry, chemical physics and biophysical chemistry. To be suitable for publication in PCCP, articles must include significant innovation and/or insight into physical chemistry; this is the most important criterion that reviewers and Editors will judge against when evaluating submissions. The journal has a broad scope and welcomes contributions spanning experiment, theory, computation and data science. Topical coverage includes spectroscopy, dynamics, kinetics, statistical mechanics, thermodynamics, electrochemistry, catalysis, surface science, quantum mechanics, quantum computing and machine learning. Interdisciplinary research areas such as polymers and soft matter, materials, nanoscience, energy, surfaces/interfaces, and biophysical chemistry are welcomed if they demonstrate significant innovation and/or insight into physical chemistry. Joined experimental/theoretical studies are particularly appreciated when complementary and based on up-to-date approaches.














