Nitric oxide activation by caa3 oxidoreductase from Thermus thermophilus

Literature Information

Publication Date 2015-03-23
DOI 10.1039/C5CP01013F
Impact Factor 3.676
Authors

Takehiro Ohta, Tewfik Soulimane, Teizo Kitagawa, Constantinos Varotsis


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Abstract

Visible and UV-resonance Raman spectroscopy was employed to investigate the reaction of NO with cytochrome caa3 from Thermus thermophilus. We show the formation of the hyponitrite (HO–NN–O)− bound to the heme a3 species (νNN = 1330 cm−1) forming a high spin complex in the oxidized heme a3 Fe/CuB binuclear center of caa3-oxidoreductase. In the absence of heme a3 Fe2+–NO formation, the electron required for the formation of the NN bond originates from the autoreduction of CuB by NO, producing nitrite. With the identification of the hyponitrite intermediate the hypothesis of a common phylogeny of aerobic respiration and bacterial denitrification is fully supported and the mechanism for the 2e−/2H+ reduction of NO to N2O can be described with more certainty.

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Physical Chemistry Chemical Physics

Physical Chemistry Chemical Physics
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