A specific UPLC-ESI-MS/MS method for analysis of cyadox and its three main metabolites in fish samples

Literature Information

Publication Date 2011-12-15
DOI 10.1039/C1AY05523B
Impact Factor 2.896
Authors

Linli Cheng, Zhanhui Wang, Jianzhong Shen, Linxia Li, Haixia Wu, Suxia Zhang


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Abstract

A rapid and sensitive ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed for the determination of cyadox (CYX) and its three major metabolites, quinoxaline-2-carboxylic acid (QCA), 1-desoxycyadox (1-DCYX) and 1,4-bisdesoxycyadox (BDCYX) in fish. Samples were extracted with acetonitrile–0.5 M hydrochloric acid (9 + 1) and cleaned up using Oasis MAX cartridges. The total time taken for separation by UPLC was less than 5 min. The four target compounds were then determined by ESI-MS/MS. At the fortified levels of 2–50 μg kg−1 in fish, recoveries of all the compounds ranged from 80.2% to 88.2%, with a relative standard deviation of 6.8 to 14.6%. Limits of detection for CYX, QCA, 1–DCYX and BDCYX were 0.52, 1.07, 0.69 and 0.38 μg kg−1, respectively.

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Analytical Methods

Analytical Methods
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