Novel fluorescence enhancement IgE assay using a DNA aptamer
Literature Information
Publication Date
2009-03-12
DOI
10.1039/B812450G
Impact Factor
4.616
Authors
Jing-Lin He, Zai-Sheng Wu, Song-Bai Zhang, Guo-Li Shen, Ru-Qin Yu
View Original
Abstract
In this paper, we demonstrate a fluorescence immunoglobulin E (IgE) assay probe based on a DNA aptamer. A Texas red-labeled short DNA strand (T-DNA) complementary with part of the IgE aptamer sequence was used to produce the fluorescence enhancement effected upon the binding of IgE to the aptamer. Another short DNA strand labeled with dabcyl quencher (Q-DNA) complementary with part of the aptamer sequence nearby the T-DNA location was used to lower the background fluorescence. The IgE can be detected in the concentration range from 9.2 × 10−11 to 3.7 × 10−8 mol L−1 with a detection limit of 5.7 × 10−11 mol L−1.
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