Direct acoustic profiling of DNA hybridisation using HSV type 1 viral sequences
Literature Information
Publication Date
2007-09-21
DOI
10.1039/B711850C
Impact Factor
4.616
Authors
Yıldız Uludağ, Xin Li, Heather Coleman, Stacey Efstathiou, Matthew A. Cooper
View Original
Abstract
We describe the detection of specific, conserved DNA sequences of herpes simplex virus (HSV) type 1 by means of a novel, high sensitivity acoustic biosensor. Repeated assays on planar and polymeric carboxylic acid- and biotin-presenting surface chemistries enabled statistical comparison of assay specificity and sensitivity and evaluation of assayZ-factor scores. Using a three minute hybridisation with NeutrAvidin capture for signal enhancement, it was possible to detect HSV viral nucleic acids at 5.2 × 10−11 M concentration.
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