Polymeric nanoparticles for protein kinase activity

Literature Information

Publication Date 2007-01-12
DOI 10.1039/B612773H
Impact Factor 6.222
Authors

Jong-Ho Kim, Seulki Lee, Kwangmeyung Kim, Hyesung Jeon, Rang-Woon Park, In-San Kim, Kuiwon Choi, Ick Chan Kwon


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Abstract

Nanoparticles were prepared from poly-ion complexes, possessing both PEI–FITC–(PKA-specific substrate) (kemptide) and PAA–TRITC, which produce intermolecular FRET; the nanoparticles were dissociated by phosphorylation, presented a strong FITC intensity and can be applied to high-throughput screening for large chemical libraries, for drug discovery of kinase inhibitors.

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