Polymeric nanoparticles for protein kinase activity
Literature Information
Jong-Ho Kim, Seulki Lee, Kwangmeyung Kim, Hyesung Jeon, Rang-Woon Park, In-San Kim, Kuiwon Choi, Ick Chan Kwon
Nanoparticles were prepared from poly-ion complexes, possessing both PEI–FITC–(PKA-specific substrate) (kemptide) and PAA–TRITC, which produce intermolecular FRET; the nanoparticles were dissociated by phosphorylation, presented a strong FITC intensity and can be applied to high-throughput screening for large chemical libraries, for drug discovery of kinase inhibitors.
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