Sequential affinity chromatography miniaturized within a “lab-on-valve” system
Literature Information
Holger Erxleben, Jaromir Ruzicka
An automatically renewable microcolumn, subjected to operation by programmable flow, is presented and for the first time used for separation and quantification of biomolecules on Sepharose Protein A beads, with absorbance measurement at 280 nm and a detection limit of 6 ng mouse IgG µL−1.
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