6-FAM SE | CAS No. 92557-81-8

6-FAM SE

Basic Information

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CAS Number

92557-81-8

Molecular Formula

C25H15NO9

Molecular Weight

473.4 g/mol

Chemical Classification

Basic Chemical Raw Materials Organic Chemicals Carboxylic Acids

Basic Chemical Raw Materials Organic Chemicals Esters

Basic Chemical Raw Materials Organic Chemicals Ethers

Basic Chemical Raw Materials Organic Chemicals Heterocyclic Compounds

Pharmaceutical Chemicals Pharmaceutical Intermediates Heterocyclic Intermediates

Fine Chemicals Dyes and Pigments Organic Dyes

Fine Chemicals Surfactants Anionic Surfactants

Laboratory Chemicals Research Chemicals Biochemical Reagents

Basic Physical Properties

Boiling Point

812.2°C at 760 mmHg

Density

1.7400

SMILES

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C1CC(=O)N(C1=O)OC(=O)C2=CC3=C(C=C2)C(=O)OC34C5=C(C=C(C=C5)O)OC6=C4C=CC(=C6)O

InChI

InChI=1S/C25H15NO9/c27-13-2-5-16-19(10-13)33-20-11-14(28)3-6-17(20)25(16)18-9-12(1-4-15(18)24(32)34-25)23(31)35-26-21(29)7-8-22(26)30/h1-6,9-11,27-28H,7-8H2

InChIKey

VDABVNMGKGUPEY-UHFFFAOYSA-N

LogP

2.886600000000002

Topological Polar Surface Area

139.67000000000002 Å²

Hydrogen Bond Donor/Acceptor

2 / 10

Complexity

894

Safety Information

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Hazard Statement

H302 Harmful if swallowed
Acute toxicity, oral

H315 Causes skin irritation
Skin corrosion/irritation

H319 Causes serious eye irritation
Serious eye damage/eye irritation

Sensitiveness

Sensitive to light

Synonyms & References

English

6-Carboxyfluorescein-NHS
MFCD00077323
DTXSID20376335
BCP14865
HY-15939
Carboxyfluorescein succinimidyl ester
R01-0025
6-Carboxyfluorescein succinimidyl ester
1-[(3',6'-dihydroxy-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-6-carbonyl)oxy]pyrrolidine-2,5-dione
SCHEMBL334086
(S)-2-CYCLOPENTYLOXYCARBONYLAMINO-3,3-DIMETHYL-BUTYRICACID
6-CarboxyfluoresceinN-hydroxysuccinimideester
A897873
SY240159
92557-81-8
BP-23900
6-Carboxyfluorescein diacetate N-succinimidyl ester
6-FAM N-succinimidyl ester
6-FAMSE
CHEBI:132203
6-Carboxyfluorescein N-hydroxy succinimide ester
FAM NHS ester, 6-isomer
6-Carboxyfluorescein N-hydroxysuccinimide ester
PD012484
J-100062
F12262
6-Carboxyfluorescein N-succinimidyl ester
2,5-dioxopyrrolidin-1-yl 3',6'-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-6-carboxylate
VDABVNMGKGUPEY-UHFFFAOYSA-N
OSu-FAM
(2,5-dioxopyrrolidin-1-yl) 3',6'-dihydroxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-carboxylate

MDL Number

MFCD00077323

Customs Code

2925290090

FAQ

What are the physical and chemical properties of 6-FAM SE (CAS: 92557-81-8)?

6-FAM SE (CAS: 92557-81-8) is a fluorescent dye with a molecular weight of approximately 411.4 g/mol. It has a melting point of around 210°C and is soluble in organic solvents like DMSO and ethanol. It exhibits high fluorescence efficiency and is often used in biochemistry and molecular biology. Chemically, it is reactive towards nucleophiles and can be used in various labeling applications.

How is 6-FAM SE (CAS: 92557-81-8) typically synthesized?

6-FAM SE (CAS: 92557-81-8) is synthesized via a multi-step process starting with the coupling of 6-fluorofluorescein to a suitable amino compound. The synthesis involves cross-coupling reactions and typically uses catalysts like Pd(II) complexes. The product is isolated and purified via column chromatography, yielding a fluorescent dye with high purity.

What industries use 6-FAM SE (CAS: 92557-81-8)?

6-FAM SE (CAS: 92557-81-8) is widely used in the pharmaceutical, biotechnology, and research industries. It is particularly important in molecular biology, where it serves as a labeling agent for nucleic acids, proteins, and other biomolecules. Its applications include fluorescence microscopy, flow cytometry, and DNA sequencing.

What precautions should be taken when handling 6-FAM SE (CAS: 92557-81-8)?

When handling 6-FAM SE (CAS: 92557-81-8), it is important to wear appropriate personal protective equipment (PPE), including gloves and safety goggles. Work should be conducted in a fume hood to minimize inhalation risks. Spills should be cleaned up immediately, and proper waste disposal procedures should be followed. Always refer to the Safety Data Sheet (SDS) for detailed handling and safety information.