Gold nanoparticle-based immunochromatographic assay for rapid detection of imazalil

Literature Information

Publication Date 2023-10-06
DOI 10.1039/D3NH00371J
Impact Factor 10.989
Authors


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Abstract

Imazalil (IMZ) is a commonly used fungicide for controlling fungus in agriculture, leaving residual IMZ in crops that could be hazardous to human health. In this work, we designed IMZ haptens for mice immunization and prepared sensitive monoclonal antibody (mAb) against IMZ. The subtype of anti-IMZ mAb is IgG2a. It possessed a half inhibition concentration (IC50) of 0.95 ng mL−1 and showed no cross-reactivity against other chemicals in ic-ELISA. Taking advantage of the mAb, we developed a gold nanoparticle-based immunochromatographic assay (GICA) for the rapid detection of IMZ in grapes and tomatoes. The assay gave a visual limit of detection (vLOD) of 25 ng g−1 and cut-off value of 500 ng g−1 in both samples. According to the calibration curves, the calculated LOD were 4.12 ng g−1 and 4.70 ng g−1 in grapes and tomatoes, respectively. The recovery rates of IMZ ranged from 84.7% to 104.4% with variation coefficients (CVs) of 5.7–11.8% in spiked samples, indicating a potent practicability of the GICA. The whole GICA process took 30 min. Therefore, the developed assay can be used for on-site detection and quantitation of IMZ in grape and tomato samples.

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Nanoscale Horizons

Nanoscale Horizons
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Nanoscale Horizons is a leading journal for the publication of exceptionally high-quality, innovative nanoscience and nanotechnology. The journal places an emphasis on original research that demonstrates a new concept or a new way of thinking (a conceptual advance), rather than primarily reporting technological improvements. However, outstanding articles featuring truly breakthrough developments such as record performance alone may also be published in the journal. For work to be published it must be of significant general interest to our community-spanning readership. Topics covered in the journal include, but are not limited to: Synthesis of nanostructured and nanoscale materials Quantum materials 2D materials Layered materials Layered quantum materials Characterisation of functional nanoscale materials and bio-assemblies Properties of nanoscale materials Self-assembly and molecular organisation Complex hybrid nanostructures Nanocomposites, nanoparticles, nanocrystalline materials, and nanoclusters Nanotubes, molecular nanowires and nanocrystals Molecular nanoscience Nanocatalysis Theoretical modelling Single-molecules Plasmonics Nanoelectronics and molecular electronics Nanophotonics Nanochips, nanosensors, nanofluidics and nanofabrication Carbon-based nanoscale materials and devices Biomimetic materials Nanobiotechnology/bionanomaterials Nanomedicine Regulatory approaches and risk assessment

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