Engineering semi-permeable giant liposomes

Literature Information

Publication Date 2023-10-31
DOI 10.1039/D3CC04039A
Impact Factor 6.222
Authors

Sreelakshmi Radhakrishnan, Samir Nandi


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Abstract

Giant unilamellar vesicles (GUVs) with a semi-permeable nature are prerequisites for constructing synthetic cells. Here we engineer semi-permeable GUVs by the inclusion of DOTAP lipid in vesicles. Diffusion of molecules of different charge and size across GUVs are reported. Control over size-selective permeability is demonstrated by modulating the DOTAP lipid composition in different lipid systems without reconstituting membrane proteins. Such semi-permeable GUVs have immense applications for constructing synthetic cells.

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