A highly specific and flexible detection assay using collaborated actions of DNA-processing enzymes for identifying multiple gene expression signatures in breast cancer
Literature Information
Dain Kim, Jiyoung Lee, Jueun Han, Jaewoo Lim
Most nucleic acid biosensors employ nucleic acid-processing enzymes to bind, degrade, splice, synthesize, and modify nucleic acids. Utilizing their unique substrate preference, binding mode, and catalytic activity is of great importance in designing nucleic acid biosensors. Combination with DNA-processing enzymes enables them to transform into a new generation of molecular diagnostics tools with enhanced selectivity and sensitivity and reduced reaction time. Here, we report an isothermal amplification strategy by coemploying a structure-specific endonuclease (flap endonuclease 1, FEN1) and a strand-displacing DNA polymerase (Bst DNA polymerase) to detect long RNA targets. This approach couples the FEN1-driven invasive cleavage reaction with toehold-mediated rolling circle amplification (iFEN–tRCA), enabling the highly selective and rapid detection of long RNA targets and offering a detection limit below 10 pM within 1 h. We used two targets, such as human epidermal growth factor receptor 2 (HER2, encoded by ERBB2) and dopamine- and cyclic AMP-regulated phosphoprotein (DARPP, encoded by PPP1R1B), associated with prognosis or response to anticancer therapy. We demonstrated the feasibility and quantitative capability of the iFEN–tRCA assay by assessing the expression of two RNA transcripts (ERBB2 and PPP1R1B) with total RNA extracts purified from human breast cancer cells. Therefore, we envision that the developed assay will provide a suitable prognostic and diagnostic tool for identifying appropriate patients for HER2-targeted therapy and predicting the clinical outcome and occurrence of metastasis relapse in breast cancer.
Recommended Journals

Journal of Natural Medicines

Organic Process Research & Development

New Journal of Chemistry

Current Opinion in Colloid & Interface Science

Russian Journal of Applied Chemistry

Russian Journal of Bioorganic Chemistry

Journal of Peptide Science

Russian Chemical Bulletin

Russian Journal of Coordination Chemistry

Crystallography Reports
Related Literature
First-principles calculations of the BeO monolayer with chemical functionalization
Hanlu Liu, Kehan Feng, Haiming Lu, Xiangkang Meng
DOI: 10.1039/D1CP05640A
Spin–orbit coupling and the fine optical structure of chiral helical polymers
Mengzhao Du, Xuan Liu, Shijie Xie
DOI: 10.1039/D2CP01092E
Impact of Bi doping on nonradiative carrier recombination in CsPbI3
Jiajia Zhang, Chenggen Xie, Lijuan Chen
DOI: 10.1039/D1CP05552F
Predicted structures and superconductivity of LiYHn (n = 5–10) under high pressure
Tao Gao, Shiyin Ma, Xiaoqiu Ye
DOI: 10.1039/D2CP00059H
Ionic migration induced loss analysis of perovskite solar cells: a poling study
Xue Zheng, Pingping Liu, Jie Zhang, Hongfei Zhou, Ming Chen, Weimin Li, Boyuan Huang, Huan Wang, Chunlei Yang
DOI: 10.1039/D1CP05450C
Molecular insights into the allosteric coupling mechanism between an agonist and two different transducers for μ-opioid receptors
Fuhui Zhang, Yuan Yuan, Yichi Chen, Jianfang Chen, Yanzhi Guo, Xuemei Pu
DOI: 10.1039/D1CP05736G
Performance of the nitrogen reduction reaction on metal bound g-C6N6: a combined approach of machine learning and DFT
Moumita Mukherjee, Sayan Dutta, Madhusudan Ghosh, Partha Basuchowdhuri, Ayan Datta
DOI: 10.1039/D2CP01901A
Absolute determination of chemical kinetic rate constants by optical tracking the reaction on the second timescale using cavity-enhanced absorption spectroscopy
Hongming Yi, Tao Wu, Amélie Lauraguais, Cecile Coeur, Alexandre Tomas, Hongbo Fu, Xiaoming Gao, Weidong Chen
DOI: 10.1039/D2CP00206J
Nucleobase-containing polymer architectures controlled by supramolecular interactions: the key to achieve biomimetic platforms with various morphologies
Laura Vasilica Arsenie, Vincent Ladmiral, Patrick Lacroix-Desmazes, Sylvain Catrouillet
DOI: 10.1039/D2PY00920J
A transferable prediction model of molecular adsorption on metals based on adsorbate and substrate properties
Paolo Restuccia, Ehsan A. Ahmad, Nicholas M. Harrison
DOI: 10.1039/D2CP01572B
You might also like
What precautions should be taken when handling lithium chloride hydrate (1:1:1) (CAS: 16712-20-2)?
When handling lithium chloride hydrate (1:1:1) (CAS: 16712-20-2), it is importan...
Is 4-(4H-1,2,4-Triazol-4-yl)piperidine (CAS: 690261-92-8) safe?
4-(4H-1,2,4-Triazol-4-yl)piperidine is generally considered safe for use in phar...
How should waste containing 1,3-Thiazole-2-carboxamide (CAS: 16733-85-0) be handled?
Waste containing 1,3-Thiazole-2-carboxamide (CAS: 16733-85-0) should be collecte...
What regulatory guidelines apply to 5-(Difluoromethyl)-2-fluorobenzonitrile (CAS: 934175-58-3)?
5-(Difluoromethyl)-2-fluorobenzonitrile (CAS: 934175-58-3) is subject to regulat...
How is Methyl 3-acetamido-2-thiophenecarboxylate (CAS: 22288-79-5) typically synthesized?
Methyl 3-acetamido-2-thiophenecarboxylate can be synthesized by the reaction of ...
What is 4-Isoquinolinecarbonitrile (CAS: 34846-65-6)?
4-Isoquinolinecarbonitrile is a chemical compound with the CAS number 34846-65-6...
How should Methyl 1H-1,2,3-triazole-4-carboxylate (CAS: 877309-59-6) be stored?
Store Methyl 1H-1,2,3-triazole-4-carboxylate (CAS: 877309-59-6) in a cool, dry p...
What regulatory guidelines apply to 6-Bromo[1,3]thiazolo[5,4-b]pyridin-2-amine (CAS: 1160791-13-8)?
6-Bromo[1,3]thiazolo[5,4-b]pyridin-2-amine (CAS: 1160791-13-8) is subject to the...
Is (2S,3S)-2-Ammonio-3-(3,4-dihydroxyphenyl)-3-hydroxypropanoate (CAS: 23651-95-8) safe?
(2S,3S)-2-Ammonio-3-(3,4-dihydroxyphenyl)-3-hydroxypropanoate (CAS: 23651-95-8) ...
What are the physical and chemical properties of 7-bromo-3-methyl-3,4-dihydroquinazolin-4-one (CAS: 1293987-84-4)?
7-Bromo-3-methyl-3,4-dihydroquinazolin-4-one is a solid with a crystalline form....
Source Journal
Analyst

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

![6,6-Dimethylbicyclo[3.1.1]hept-2-ene-2-carbaldehyde structure 6,6-Dimethylbicyclo[3.1.1]hept-2-ene-2-carbaldehyde structure](https://static.chemtradehub.com/structs/564/564-94-3-e746.webp)
![tert-butyl 8-benzyl-2,8-diazaspiro[4.5]decane-2-carboxylate structure tert-butyl 8-benzyl-2,8-diazaspiro[4.5]decane-2-carboxylate structure](https://static.chemtradehub.com/structs/336/336191-16-3-bb55.webp)

