Selective binding of nucleosides to gapped DNA duplex revealed by orientation and distance dependence of FRET
Literature Information
Publication Date
2019-05-16
DOI
10.1039/C9OB00946A
Impact Factor
3.876
Authors
Hiromu Kashida, Yuta Kokubo, Koki Makino, Hiroyuki Asanuma
View Original
Abstract
Herein we used orientation and distance dependence of Förster resonance energy transfer (FRET) to analyze the binding of nucleosides to a gapped DNA duplex. Binding isotherms and information on the structures of the complexes were obtained by monitoring FRET between pyrene and perylene, which were introduced into the DNA through D-threoninol. FRET efficiency significantly changed upon formation of a duplex with a 1-nucleotide gap and a nucleoside. The FRET plot indicated that the complex has a double helical structure similar to a nicked duplex. Cooperative binding of two nucleosides to a duplex with a 2-nucleotide gap was also revealed using FRET. Various drug-nucleic acids interactions could be investigated using this sensitive and facile method.
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Source Journal
Organic & Biomolecular Chemistry
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