Detection and identification of O-GlcNAc-modified proteins using 6-azido-6-deoxy-N-acetyl-galactosamine

Literature Information

Publication Date 2019-04-06
DOI 10.1039/C9OB00516A
Impact Factor 3.876
Authors

Jianshuang Guo, Guoqiang Zhang, Jing Ma, Caili Zhao, Qingqing Xue, Jiyan Wang, Wenjie Liu, Kaihui Liu, Haifeng Wang, Ning Liu, Qitao Song, Jing Li


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Abstract

An unnatural monosaccharide with a C6-azide, Ac36AzGalNAc, has been developed as a potent and selective probe for O-GlcNAc-modified proteins. Combined with click chemistry, we demonstrate that Ac36AzGalNAc can robustly label O-GlcNAc glycosylation in a wide range of cell lines. Meanwhile, cell imaging and LC-MS/MS proteomics verify its selective activity on O-GlcNAc. More importantly, the protocol presented here provides a general methodology for tracking, capturing and identifying unnatural monosaccharide modified proteins in cells or cell lysates.

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