Detection and identification of O-GlcNAc-modified proteins using 6-azido-6-deoxy-N-acetyl-galactosamine
Literature Information
Jianshuang Guo, Guoqiang Zhang, Jing Ma, Caili Zhao, Qingqing Xue, Jiyan Wang, Wenjie Liu, Kaihui Liu, Haifeng Wang, Ning Liu, Qitao Song, Jing Li
An unnatural monosaccharide with a C6-azide, Ac36AzGalNAc, has been developed as a potent and selective probe for O-GlcNAc-modified proteins. Combined with click chemistry, we demonstrate that Ac36AzGalNAc can robustly label O-GlcNAc glycosylation in a wide range of cell lines. Meanwhile, cell imaging and LC-MS/MS proteomics verify its selective activity on O-GlcNAc. More importantly, the protocol presented here provides a general methodology for tracking, capturing and identifying unnatural monosaccharide modified proteins in cells or cell lysates.
Related Literature
Stability of 2′,3′ and 3′,5′ cyclic nucleotides in formamide and in water: a theoretical insight into the factors controlling the accumulation of nucleic acid building blocks in a prebiotic pool
Giuseppe Cassone, Franz Saija, Ernesto Di Mauro, A. Marco Saitta, Judit E. Šponer
DOI: 10.1039/C6CP07993H
Size-dependent one-photon- and two-photon-pumped amplified spontaneous emission from organometal halide CH3NH3PbBr3 perovskite cubic microcrystals
Zhen-Yu Zhang, Hai-Yu Wang, Yan-Xia Zhang, Kai-Jiao Li, Xue-Peng Zhan, Bing-Rong Gao, Qi-Dai Chen, Hong-Bo Sun
DOI: 10.1039/C6CP07522C
Infrared characterisation of acetonitrile and propionitrile aerosols under Titan's atmospheric conditions
C. Ennis, R. Auchettl, M. Ruzi, E. G. Robertson
DOI: 10.1039/C6CP08110J
Transient photocyclization in ruthenium(ii) polypyridine complexes of indolamines
G. Carrone, L. Zayat, L. D. Slep, R. Etchenique
DOI: 10.1039/C6CP06580E
Second-order nonlinear optical properties of bulk GeC polytypes, g-GeC and corresponding nanotubes: first-principles calculations
Dongshan Wei
DOI: 10.1039/C6CP07573H
Ferromagnetism in nitrogen-doped BaO: a self-interaction corrected DFT study
Elisa Albanese, Gianfranco Pacchioni
DOI: 10.1039/C6CP07535E
Observation of partial reduction of manganese in the lithium rich layered oxides, 0.4Li2MnO3–0.6LiNi1/3Co1/3Mn1/3O2, during the first charge
Hyung Cheoul Shim, Donghan Kim, Dongwook Shin, Seungmin Hyun, Chang-Su Woo, Taehwan Yu, Jae-Pyoung Ahn
DOI: 10.1039/C6CP07574F
In situ X-ray absorption near edge structure studies and charge transfer kinetics of Na6[V10O28] electrodes
Chun-Jern Pan, Ali Haider, Rami Al-Oweini, Ming-Hsien Lin, Ulrich Kortz
DOI: 10.1039/C6CP05768C
Probing nonlinear optical coefficients in self-assembled peptide nanotubes
Soma Khanra, Kartik Ghosh, Fabio F. Ferreira, Wendel A. Alves, Francesco Punzo, Ping Yu, Suchismita Guha
DOI: 10.1039/C6CP07879F
Reactions driving conformational movements (molecular motors) in gels: conformational and structural chemical kinetics
DOI: 10.1039/C6CP06735B
You might also like
Is 2-(2-chloroacetamido)-3-phenylpropanoic acid (CAS: 7765-11-9) safe?
2-(2-Chloroacetamido)-3-phenylpropanoic acid (CAS: 7765-11-9) is generally consi...
Is 2-(Benzyloxy)-5-bromobenzoic acid (CAS: 62176-31-2) safe?
2-(Benzyloxy)-5-bromobenzoic acid can be handled safely if appropriate precautio...
What is (4-Methyl-1,2,5-oxadiazol-3-yl)methanamine hydrochloride (CAS: 1159825-48-5)?
(4-Methyl-1,2,5-oxadiazol-3-yl)methanamine hydrochloride is a chemical compound ...
What is 2-(5-Hexylthiophen-2-yl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane (CAS: 917985-54-7)?
2-(5-Hexylthiophen-2-yl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane (CAS: 917985-54...
Are there alternatives to 4-(8-Methyl-9H-1,3-dioxolo[4,5-h][2,3]benzodiazepin-5-yl)benzenamine (CAS: 102771-26-6) in synthesis?
While 4-(8-Methyl-9H-1,3-dioxolo[4,5-h][2,3]benzodiazepin-5-yl)benzenamine (CAS:...
What is the market or research trend for tert-butyl 3-hydroxy-4,5,7,8-tetrahydro-2H-pyrazolo[3,4-d]azepine-6-carboxylate (CAS: 851376-80-2)?
The market for tert-butyl 3-hydroxy-4,5,7,8-tetrahydro-2H-pyrazolo[3,4-d]azepine...
How should waste containing 3,5-Diamino-1H-pyrazole-4-carbonitrile (CAS: 6844-58-2) be handled?
Waste containing 3,5-Diamino-1H-pyrazole-4-carbonitrile (CAS: 6844-58-2) should ...
How is (6-Fluoro-3-pyridinyl)boronic acid (CAS: 351019-18-6) typically synthesized?
(6-Fluoro-3-pyridinyl)boronic acid can be synthesized through the reaction of 6-...
What industries use Dibenzyl carbonimidoylbiscarbamate (CAS: 10065-79-9)?
Dibenzyl carbonimidoylbiscarbamate (CAS: 10065-79-9) finds applications in vario...
What is the market or research trend for (beta,beta,2,3,4,5,6-~2~H_7_)Phenylalanine (CAS: 74228-83-4)?
The market for (beta,beta,2,3,4,5,6-~2~H_7_)Phenylalanine (CAS: 74228-83-4) is g...
Source Journal
Organic & Biomolecular Chemistry

Organic & Biomolecular Chemistry (OBC) publishes original and high impact research and reviews in organic chemistry. We welcome research that shows new or significantly improved protocols or methodologies in total synthesis, synthetic methodology or physical and theoretical organic chemistry as well as research that shows a significant advance in the organic chemistry or molecular design aspects of chemical biology, catalysis, supramolecular and macromolecular chemistry, theoretical chemistry, mechanism-oriented physical organic chemistry, medicinal chemistry or natural products. Articles published in the journal should report new work which makes a highly-significant impact in the field. Routine and incremental work is generally not suitable for publication in the journal. More details about key areas of our scope are below. In all cases authors should include in their article clear rationale for why their research has been carried out.












![3,7-Di(1,1':3',1''-terphenyl-5'-yl)-10,11,12,13-tetrahydrodiindeno[7,1-de:1',7'-fg][1,3,2]dioxaphosphocin-5-ol 5-oxide structure 3,7-Di(1,1':3',1''-terphenyl-5'-yl)-10,11,12,13-tetrahydrodiindeno[7,1-de:1',7'-fg][1,3,2]dioxaphosphocin-5-ol 5-oxide structure](https://static.chemtradehub.com/structs/135/1352810-38-8-3f10.webp)

