A cell-permeant small molecule for the super-resolution imaging of the endoplasmic reticulum in live cells
Literature Information
Adiki Raja Sekhar, Bhagaban Mallik, Vimlesh Kumar, Jeyaraman Sankar
The endoplasmic reticulum (ER) constitutes about half of the total membrane of a eukaryotic cell, and defects in the ER have been shown to be linked with a variety of diseases. To investigate these underlying mechanisms in detail, the specific labelling of the ER for high-resolution long-term live-imaging can serve as an important tool. Here, we report the identification of a stimulated emission depletion (STED) microscopy-compatible BODIPY derivative (NH2-BODIPY) to selectively image the ER. In contrast to the conventional ER-Trackerâ„¢ dye, NH2-BODIPY selectively labels the ER at a much lower concentration with no detectable cytotoxicity. Conventional imaging agents are often unstable under the intense light field used for STED microscopy, but NH2-BODIPY is stable due to its robust structure and therefore it has the potential to be widely exploited for ER imaging.
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Organic & Biomolecular Chemistry

Organic & Biomolecular Chemistry (OBC) publishes original and high impact research and reviews in organic chemistry. We welcome research that shows new or significantly improved protocols or methodologies in total synthesis, synthetic methodology or physical and theoretical organic chemistry as well as research that shows a significant advance in the organic chemistry or molecular design aspects of chemical biology, catalysis, supramolecular and macromolecular chemistry, theoretical chemistry, mechanism-oriented physical organic chemistry, medicinal chemistry or natural products. Articles published in the journal should report new work which makes a highly-significant impact in the field. Routine and incremental work is generally not suitable for publication in the journal. More details about key areas of our scope are below. In all cases authors should include in their article clear rationale for why their research has been carried out.












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