Nicotinamide adenine dinucleotide detection based on silver nanoclusters stabilized by a dumbbell-shaped probe
Literature Information
Hong-Ya Wang, Jin-Liang Ma, Bin-Cheng Yin
We have developed a novel method for detecting nicotinamide adenine dinucleotide (NAD+) based on fluorescent silver nanoclusters (AgNCs) stabilized by a dumbbell-shaped DNA template containing two cytosine-loops joined in a dsDNA stem. The design involves two types of components: a dumbbell-shaped DNA template and three enzymes. In the presence of NAD+ as a cofactor, Escherichia coli DNA ligase (E.coli DNA ligase) catalyzes template ligation to generate a sealed (no terminal nucleotides) dumbbell-shaped structure, preventing digestion by exonuclease III (Exo III) and exonuclease I (Exo I). The loop regions of the intact template serve as sites for the deposition of highly fluorescent AgNCs. In the absence of NAD+, the ligation reaction does not occur, and the unsealed dumbbell-shaped template is digested into mononucleotides via cooperation of Exo III and Exo I. The destruction of the DNA template results in the agglomeration of AgNCs into silver nanoparticles with low fluorescence. The fluorescence enhancement depends on the ligation and digestion of the DNA template, allowing quantitative detection of NAD+ in the range of 0.5 nM–5000 nM with a detection limit of ∼0.25 nM.
Related Literature
Spectroscopic and theoretical investigations of adenosine 5′-diphosphate and adenosine 5′-triphosphate dianions in the gas phase
Paul E. Crider, Matthias Vonderach, Patrick Weis
DOI: 10.1039/C2CP43808A
Characterization and control of the electronic properties of a NiO based dye sensitized photocathode
Idan Hod, Zion Tachan, Menny Shalom, Arie Zaban
DOI: 10.1039/C3CP50242B
Mesoscopic modelling of frustration in microemulsions
Magali Duvail, Jean-François Dufrêche, Lise Arleth, Thomas Zemb
DOI: 10.1039/C3CP43981J
Modeling environment effects on spectroscopies through QM/classical models
Benedetta Mennucci
DOI: 10.1039/C3CP44417A
Non-bonding interactions and internal dynamics in CH2F2⋯H2CO: a rotational and model calculations study
Qian Gou, Gang Feng, Luca Evangelisti, Alberto Lesarri, Emilio J. Cocinero, Walther Caminati
DOI: 10.1039/C3CP50306B
Ultrahigh-efficiency photocatalysts based on mesoporous Pt–WO3 nanohybrids
Zhuang Liu, Hao Zhang, Jinghong Li, Junhong Chen
DOI: 10.1039/C3CP50647A
Laser directed lithography of asymmetric graphene ribbons on a polydimethylsiloxane trench structure
Yi Yang, Dan Xie, Tian-Ling Ren, Yi Shu, Hui Sun, Chang-Jian Zhou, Xuan Liu, Lu-Qi Tao, Jie Ge, Cang-Hai Zhang, Yuegang Zhang
DOI: 10.1039/C3CP50538C
QM/MM simulations of vibrational spectra of bacteriorhodopsin and channelrhodopsin-2
Kai Welke, Hiroshi C. Watanabe, Tino Wolter, Marcus Elstner
DOI: 10.1039/C3CP44181D
Photoinduced energy and charge transfer in a p-phenylene-linked dyad of boron dipyrromethene and monostyryl boron dipyrromethene
Roel Menting, Jian-Yong Liu, Ying-Si Huang, Dennis K. P. Ng, Beate Röder
DOI: 10.1039/C3CP50576F
Photoelectrical properties and the electronic structure of Tl1−xIn1−xSnxSe2 (x = 0, 0.1, 0.2, 0.25) single crystalline alloys
G. E. Davydyuk, H. Kamarudin, G. L. Myronchuk, S. P. Danylchuk, A. O. Fedorchuk, L. V. Piskach, M. Yu. Mozolyuk, O. V. Parasyuk
DOI: 10.1039/C3CP50836F
You might also like
What is the market or research trend for N-(4-Methoxybenzyl)-2-pyridinamine (CAS: 52818-63-0)?
N-(4-Methoxybenzyl)-2-pyridinamine (CAS: 52818-63-0) is increasingly being used ...
What precautions should be taken when handling Ethyl 4-(2-chlorophenyl)-1,3-thiazole-2-carboxylate (CAS: 1050507-06-6)?
When handling Ethyl 4-(2-chlorophenyl)-1,3-thiazole-2-carboxylate, appropriate p...
What regulatory guidelines apply to diethyldiselane (CAS: 628-39-7)?
Diethyldiselane (CAS: 628-39-7) is classified under the Globally Harmonized Syst...
What is the market or research trend for oxocopper (CAS: 12053-18-8)?
The market for oxocopper (CAS: 12053-18-8) is primarily driven by its use in cat...
What is the market or research trend for 5-{[(2-Methyl-2-propanyl)oxy]carbonyl}-5-azaspiro[2.4]heptane-7-carboxylic acid?
The market for 5-{[(2-Methyl-2-propanyl)oxy]carbonyl}-5-azaspiro[2.4]heptane-7-c...
What is 2-(1-Pyrrolidinyl)-4-pyridinamine (CAS: 35981-63-6)?
2-(1-Pyrrolidinyl)-4-pyridinamine is a chemical compound with the CAS number 359...
What are the physical and chemical properties of 2-(3-Pyridinyl)-1-azabicyclo[2.2.2]octane (CAS: 91556-75-1)?
2-(3-Pyridinyl)-1-azabicyclo[2.2.2]octane (CAS: 91556-75-1) is a crystalline sol...
How is (S)-Alpha-allyl-proline hydrochloride (CAS: 129704-91-2) typically synthesized?
(S)-Alpha-allyl-proline hydrochloride is usually synthesized via a Wittig reacti...
What is 3-Methyl-1,2-oxazole-5-carboxylic acid (CAS: 4857-42-5)?
3-Methyl-1,2-oxazole-5-carboxylic acid (CAS: 4857-42-5) is an organic compound w...
How is Lys-SMCC-DM1 (CAS: 1281816-04-3) typically synthesized?
Lys-SMCC-DM1 is synthesized via a multi-step process involving the coupling of S...
Source Journal
Analyst

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.












![1-[6-(1H-Imidazol-1-yl)-3-pyridinyl]methanamine structure 1-[6-(1H-Imidazol-1-yl)-3-pyridinyl]methanamine structure](https://static.chemtradehub.com/structs/914/914637-08-4-8825.webp)
![1-oxaspiro[4.4]nonan-6-one structure 1-oxaspiro[4.4]nonan-6-one structure](https://static.chemtradehub.com/structs/134/134179-01-4-e051.webp)
![N-{[(2-Methyl-2-propanyl)oxy]carbonyl}-L-methionylglycine structure N-{[(2-Methyl-2-propanyl)oxy]carbonyl}-L-methionylglycine structure](https://static.chemtradehub.com/structs/234/23446-03-9-e1e5.webp)