Nanoscale mapping of newly-synthesised phospholipid molecules in a biological cell using tip-enhanced Raman spectroscopy

Literature Information

Publication Date 2017-02-08
DOI 10.1039/C6CC10226C
Impact Factor 6.222
Authors

Naresh Kumar, Marek M. Drozdz, Haibo Jiang, Daniela M. Santos, David J. Vaux


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Abstract

Nanoscale chemical mapping of newly-synthesised phospholipid molecules inside a mammalian cell is demonstrated using tip-enhanced Raman spectroscopy (TERS) for the first time using mouse pre-adipocyte cells as a model system. Newly-synthesised membrane phospholipid distribution within a pre-adipocyte cell is mapped with <20 nm spatial resolution, overcoming the diffraction limit of confocal Raman spectroscopy via plasmonic enhancement of Raman signals at a TERS tip-apex.

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