Nonidentical intracellular drug release rates in Raman and fluorescence spectroscopic determination

Literature Information

Publication Date 2014-11-24
DOI 10.1039/C4CP04235B
Impact Factor 3.676
Authors

Erdene-Ochir Ganbold, Jinha Yoon, Doseok Kim, Sang-Woo Joo


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Abstract

Intracellular drug release rates were measured by monitoring mitoxantrone (MTX) on gold nanoparticle (AuNP) carriers by means of real-time label-free bimodal imaging with confocal Raman and fluorescence spectroscopy. The quenching nature of the MTX–AuNPs by nanometal surface energy transfer (NSET) was analyzed using the determined Stern–Volmer constant of KSV = 2.28 × 109 M−1. The amount of MTX released was estimated by both the decrease in the surface-enhanced resonance Raman scattering (SERRS) signal and the increase in the fluorescence intensity. Both SERRS and NSET provide quantitative relationships between the spectral intensities of MTX concentrations in solution. Inside live cells, the signal decay profiles of the drug release from AuNPs appeared to be faster at the beginning of the bond-breaking drug release for the SERRS (R−12) than the recovery time of the NSET (R−4 or R−6). In the first 45 min, a rather fast decay rate k of 0.0252 min−1 with a short half-life t1/2 of 27.5 min was observed, whereas the rate became significantly slower in a diffusion process, 0.0093 min−1 with a longer half-life of 101.4 min, after 45 min.

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Physical Chemistry Chemical Physics

Physical Chemistry Chemical Physics
CiteScore: 5.5
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Physical Chemistry Chemical Physics (PCCP) is an international journal co-owned by 19 physical chemistry and physics societies from around the world. This journal publishes original, cutting-edge research in physical chemistry, chemical physics and biophysical chemistry. To be suitable for publication in PCCP, articles must include significant innovation and/or insight into physical chemistry; this is the most important criterion that reviewers and Editors will judge against when evaluating submissions. The journal has a broad scope and welcomes contributions spanning experiment, theory, computation and data science. Topical coverage includes spectroscopy, dynamics, kinetics, statistical mechanics, thermodynamics, electrochemistry, catalysis, surface science, quantum mechanics, quantum computing and machine learning. Interdisciplinary research areas such as polymers and soft matter, materials, nanoscience, energy, surfaces/interfaces, and biophysical chemistry are welcomed if they demonstrate significant innovation and/or insight into physical chemistry. Joined experimental/theoretical studies are particularly appreciated when complementary and based on up-to-date approaches.

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