Enhanced-fluorescence correlation spectroscopy at micro-molar dye concentration around a single gold nanorod

Literature Information

Publication Date 2014-09-01
DOI 10.1039/C4CP03057E
Impact Factor 3.676
Authors

Saumyakanti Khatua, Haifeng Yuan, Michel Orrit


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Abstract

Fluorescence correlation spectroscopy (FCS) is a standard tool for studying diffusion of molecules in solution, but is limited to low analyte concentrations, in the range between 10 pM and 1 nM. Such concentration limitations can be overcome by using a plasmonic nanoantenna which confines the electric field of excitation light into a tiny volume near its surface and thereby reduces the effective excitation volume by several orders of magnitude. Here we demonstrate successful FCS measurements on a 1 μM solution of crystal violet (CV) dye in glycerol using a gold nanorod antenna. Our correlation analysis yields two components: (i) a slow component with correlation time of about 100 ms, which is attributed to sticking and bleaching of the dye, and (ii) a fast component of about 1 ms, which could arise from dye diffusion through the near-field of the nanorod and/or from blinking due to intersystem crossing or photochemistry.

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Physical Chemistry Chemical Physics

Physical Chemistry Chemical Physics
CiteScore: 5.5
Self-citation Rate: 10.3%
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Physical Chemistry Chemical Physics (PCCP) is an international journal co-owned by 19 physical chemistry and physics societies from around the world. This journal publishes original, cutting-edge research in physical chemistry, chemical physics and biophysical chemistry. To be suitable for publication in PCCP, articles must include significant innovation and/or insight into physical chemistry; this is the most important criterion that reviewers and Editors will judge against when evaluating submissions. The journal has a broad scope and welcomes contributions spanning experiment, theory, computation and data science. Topical coverage includes spectroscopy, dynamics, kinetics, statistical mechanics, thermodynamics, electrochemistry, catalysis, surface science, quantum mechanics, quantum computing and machine learning. Interdisciplinary research areas such as polymers and soft matter, materials, nanoscience, energy, surfaces/interfaces, and biophysical chemistry are welcomed if they demonstrate significant innovation and/or insight into physical chemistry. Joined experimental/theoretical studies are particularly appreciated when complementary and based on up-to-date approaches.

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