A rapid and convenient method for detecting a broad spectrum of malignant cells from malignant pleuroperitoneal effusion of patients using a multifunctional NIR heptamethine dye
Literature Information
Ying Tian, Jing Sun, Huaijiang Yan, Zhaogang Teng, Leyong Zeng, Ying Liu, Yanjun Li, Jiandong Wang, Shouju Wang, Guangming Lu
Detection of malignant cells from malignant effusion is crucial to establish or adjust therapies of patients with cancer. The conventional qualitative detection in malignant pleuroperitoneal effusion is cytological analysis, which is time-consuming and complicated. Therefore, a faster and more convenient detection strategy is urgently needed. In this study, we report a rapid method to detect malignant cells from malignant pleuroperitoneal effusion (hydrothorax and ascites) of patients using IR-808, a tumor-targeted near-infrared (NIR) fluorescent heptamethine dye (tNRI dye), which exhibited superior labeling efficacy without specific conjugation to biomarkers. The targeted imaging performance toward malignant cells using IR-808 was confirmed by comparing with normal cells, and the fluorescence stability assay of IR-808 in malignant effusion was performed from 1 h to 48 h. In order to save time and dose, the incubation time and concentration were optimized to 10 min and 5 μM, which were used to detect malignant cells from 28 clinical samples of malignant pleuroperitoneal effusion. The results revealed that IR-808 could be internalized selectively by malignant cells of samples, and these malignant cells could be easily distinguished from normal cells under a fluorescence microscope. The positive rates between cytological analysis and the IR-808 staining method were 86% (24/28) and 79% (22/28), respectively. An excellent concordance level (Kappa = 0.752, P < 0.001) was observed between the two methods. Our results indicated that IR-808, a new NIR fluorescent heptamethine dye with unique optical imaging and tumor targeting properties, could provide a fast and simple way to detect a broad spectrum of malignant cells from malignant pleuroperitoneal effusion in patients.
Recommended Journals

Lab on a Chip

Angewandte Chemie International Edition

Environmental Toxicology and Pharmacology

Green Chemistry

Journal of Medical Biochemistry

Current Pharmaceutical Biotechnology

Faraday Discussions

Molecular Diversity

Journal of Enzyme inhibition and Medicinal Chemistry

Mini-Reviews in Medicinal Chemistry
Related Literature
Polymerization of 5-alkyl δ-lactones catalyzed by diphenyl phosphate and their sequential organocatalytic polymerization with monosubstituted epoxides
Junpeng Zhao, Nikos Hadjichristidis
DOI: 10.1039/C5PY00019J
Correction: Chemistry of aromatic polythioesters and polydithioesters
Daisuke Abe, Yuichiro Fukuda, Yuji Sasanuma
DOI: 10.1039/C6PY90024K
Tuning multiple arms for camptothecin and folate conjugations on star-shaped copolymers to enhance glutathione-mediated intracellular drug delivery
Yun Zhang, Maohua Chen, Xiaoming Luo, Hong Zhang, Chaoyu Liu, Huiyan Li, Xiaohong Li
DOI: 10.1039/C4PY01607F
A novel and facile strategy to inhibit corrosion: thiol-click synthesis of polythiols and their skinning on a metal surface to form super thick protective films
Lingxiao Gu, Qingquan Xue, Shusen Peng, Gang Wang, Jin Han, Xuedong Wu
DOI: 10.1039/C5PY01517K
The construction of single-layer two-dimensional supramolecular organic frameworks in water through the self-assembly of rigid vertexes and flexible edges
Cheng-Bin Nie, Tian-You Zhou, Qiao-Yan Qi, Jie Fu, Xiao-Zhong Wang, Liyan Dai, Yingqi Chen, Xin Zhao
DOI: 10.1039/C4PY01669F
A synthetic strategy for the preparation of sub-100 nm functional polymer particles of uniform diameter
Kato L. Killops, Pontus Lundberg, Craig J. Hawker
DOI: 10.1039/C4PY01703J
Synthesis of a polymethyl(methacrylate)-polystyrene-based diblock copolymer containing biotin for selective protein nanopatterning
B. Sasso, N. Tesson, C. Cantos
DOI: 10.1039/C5PY01601K
Tristable data storage device of soluble polyimides based on novel asymmetrical diamines containing carbazole
Jing Zhao, Ling Peng, Ya-Liang Zhu, An-min Zheng, Ying-Zhong Shen
DOI: 10.1039/C5PY02002F
(PAA-g-PS)-co-PPEGMEMA asymmetric polymer brushes: synthesis, self-assembly, and encapsulating capacity for both hydrophobic and hydrophilic agents
Binbin Xu, Guangxin Gu, Chun Feng, Xue Jiang, Jianhua Hu, Guolin Lu, Sen Zhang, Xiaoyu Huang
DOI: 10.1039/C5PY01644D
You might also like
Is 2-(2-chloroacetamido)-3-phenylpropanoic acid (CAS: 7765-11-9) safe?
2-(2-Chloroacetamido)-3-phenylpropanoic acid (CAS: 7765-11-9) is generally consi...
Is 2-(Benzyloxy)-5-bromobenzoic acid (CAS: 62176-31-2) safe?
2-(Benzyloxy)-5-bromobenzoic acid can be handled safely if appropriate precautio...
What is (4-Methyl-1,2,5-oxadiazol-3-yl)methanamine hydrochloride (CAS: 1159825-48-5)?
(4-Methyl-1,2,5-oxadiazol-3-yl)methanamine hydrochloride is a chemical compound ...
What is 2-(5-Hexylthiophen-2-yl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane (CAS: 917985-54-7)?
2-(5-Hexylthiophen-2-yl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane (CAS: 917985-54...
Are there alternatives to 4-(8-Methyl-9H-1,3-dioxolo[4,5-h][2,3]benzodiazepin-5-yl)benzenamine (CAS: 102771-26-6) in synthesis?
While 4-(8-Methyl-9H-1,3-dioxolo[4,5-h][2,3]benzodiazepin-5-yl)benzenamine (CAS:...
What is the market or research trend for tert-butyl 3-hydroxy-4,5,7,8-tetrahydro-2H-pyrazolo[3,4-d]azepine-6-carboxylate (CAS: 851376-80-2)?
The market for tert-butyl 3-hydroxy-4,5,7,8-tetrahydro-2H-pyrazolo[3,4-d]azepine...
How should waste containing 3,5-Diamino-1H-pyrazole-4-carbonitrile (CAS: 6844-58-2) be handled?
Waste containing 3,5-Diamino-1H-pyrazole-4-carbonitrile (CAS: 6844-58-2) should ...
How is (6-Fluoro-3-pyridinyl)boronic acid (CAS: 351019-18-6) typically synthesized?
(6-Fluoro-3-pyridinyl)boronic acid can be synthesized through the reaction of 6-...
What industries use Dibenzyl carbonimidoylbiscarbamate (CAS: 10065-79-9)?
Dibenzyl carbonimidoylbiscarbamate (CAS: 10065-79-9) finds applications in vario...
What is the market or research trend for (beta,beta,2,3,4,5,6-~2~H_7_)Phenylalanine (CAS: 74228-83-4)?
The market for (beta,beta,2,3,4,5,6-~2~H_7_)Phenylalanine (CAS: 74228-83-4) is g...
Source Journal
Analyst

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.
![2-(5-Bromo-1H-pyrrolo[2,3-B]pyridin-3-YL)acetic acid structure 2-(5-Bromo-1H-pyrrolo[2,3-B]pyridin-3-YL)acetic acid structure](https://static.chemtradehub.com/structs/106/1060795-03-0-0589.webp)



