Haptens, bioconjugates, and antibodies for penthiopyrad immunosensing

Literature Information

Publication Date 2014-08-26
DOI 10.1039/C4AN00828F
Impact Factor 4.616
Authors

Eric Ceballos-Alcantarilla, Antonio Abad-Fuentes, Vincenzo Aloisio, Consuelo Agulló, Antonio Abad-Somovilla, Josep V. Mercader


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Abstract

Haptens, bioconjugates, and antibodies for highly sensitive immunochemical analysis of the new-generation fungicide penthiopyrad are described. Two haptens with equivalent carboxylated linkers were prepared, and the purified active esters were efficiently coupled to proteins. The results revealed slightly different antibody-eliciting capacities for the two synthetic derivatives. All of the produced antibodies were specific for penthiopyrad, and showed affinity values in the nanomolar range.

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