Functionalized nanoscale through microscale polypeptide stabilized emulsions for display of biomolecules
Literature Information
Publication Date
2011-03-15
DOI
10.1039/C1PY00045D
Impact Factor
5.582
Authors
Jarrod A. Hanson
View Original
Abstract
We have prepared stable nano- and microscale emulsion droplets using block copolypeptide surfactants that have been functionalized with biotin. We have varied the biotin density on the droplet surfaces by mixing biotinylated with non-biotinylated surfactants, and used these to optimize the level of biotinylation for binding of NeutrAvidin. Using fluorescence microscopy and dynamic light scattering we have verified specific binding of NeutrAvidin to the biotinylated droplets and developed conditions for specific binding of biotinylated ligands to the NeutrAvidin coated droplets. These experiments outline a process for preparation of a soft, nano- through microscale sized droplet platform capable of specifically conjugating and displaying biotinylated molecules.
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Source Journal
Polymer Chemistry
CiteScore:
8.6
Self-citation Rate:
7.3%
Articles per Year:
457
Polymer Chemistry welcomes submissions in all areas of polymer science that have a strong focus on macromolecular chemistry. Manuscripts may cover a broad range of fields, yet no direct application focus is required.
Recommended Compounds
Benzyl 3-(aminomethyl)piperidine-1-carboxylate hydrochloride
CAS Number:
1186663-23-9
Molecular Formula:
C14H21ClN2O2
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