A study of alcohol-induced gelation of β-lactoglobulin with small-angle neutron scattering, neutron spin echo, and dynamic light scattering measurements

Literature Information

Publication Date 2010-02-11
DOI 10.1039/B920187D
Impact Factor 3.676
Authors

Koji Yoshida, Toshio Yamaguchi, Noboru Osaka, Hitoshi Endo, Mitsuhiro Shibayama


View Original

Abstract

Gelation of β-lactoglobulin (β-Lg) in various alcohol–water mixtures with 0.1 M (M = mol L−1) hydrochloric acid was investigated with small-angle neutron scattering (SANS), neutron spin echo (NSE), and time-resolved dynamic light scattering (TRDLS) measurements. The β-Lg in alcohol–water solutions undergoes gelation at specific alcohol concentrations where the alcohol-induced α-helical structure of β-Lg is stabilized. The SANS profiles showed that β-Lg exists as a single molecule at a low alcohol concentration. With increasing alcohol concentration, the profiles indicate a power law behavior of ∼1.7 when the samples gelate. These behaviors were observed in all alcohol–water mixtures used, but the alcohol concentrations where the SANS profiles change shift to a lower alcohol concentration region with an increase in the size of the hydrophobic group of the alcohols. Apparent diffusion constants, obtained from the intermediate scattering function (ISF) of NSE and the intensity time correlation function (ITCF) of TRDLS, mainly depend on the viscosity of alcohol–water mixtures before gelation. After gelation, on the other hand, the ISFs of gels do not change appreciably in the range of the NSE time scale, indicating the microscopically rigid structure of β-Lg gel. The ITCF functions obtained from TRDLS follow a double exponential decay type before gelation, but a logarithmic one (exponent α = 0.7) after gelation. It is most likely that the alcohol-induced gelation undergoes a similar mechanism to that for the heat-induced one at pH = 7 where β-Lg aggregates stick together to form a fractal network, although the gelation time is faster in the former than in the latter.

Related Literature

A Grand Canonical Monte Carlo study of argon adsorption/condensation in mesoporous silica glasses

Roland J.-M. Pellenq, Bernard Rousseau, Pierre E. Levitz

2001-03-08 Paper

DOI: 10.1039/B008961N

Experimental and simulation studies of the electron transfer reaction between [Ru(NH3)5pz]2+ and [Co(C2O4)3]3−

Pilar Pérez-Tejeda, Francisco-Javier Franco, Antonio Sánchez, Manuel Morillo, Claus Denk, Francisco Sánchez

2001-03-06 Paper

DOI: 10.1039/B009004M

You might also like

Compound Q&A

What are the main uses of 4-Nitrophenyl phosphate disodium salt hexahydrate (CAS: 333338-18-4)?

4-Nitrophenyl phosphate disodium salt hexahydrate is primarily used as a substra...

333338-18-44-Nitrophenyl phosph...
Compound Q&A

What are the main uses of 2-(Trifluoromethyl)-1,3-oxazole-4-carboxylic Acid (CAS: 1060816-01-4)?

2-(Trifluoromethyl)-1,3-oxazole-4-carboxylic Acid (CAS: 1060816-01-4) is widely ...

1060816-01-42-(Trifluoromethyl)-...
Compound Q&A

How should 2-Fluoro-4-biphenylcarboxylic acid (CAS: 137045-30-8) be stored?

2-Fluoro-4-biphenylcarboxylic acid should be stored in a cool, dry place at room...

137045-30-82-Fluoro-4-biphenylc...
Compound Q&A

What industries use Prednisolone-21-Carboxylic Acid (CAS: 61549-70-0)?

Prednisolone-21-Carboxylic Acid is primarily used in the pharmaceutical industry...

61549-70-0Prednisolone-21-Carb...
Compound Q&A

How should 4-(Hydrazinomethyl)-1,2,3-benzenetriol (CAS: 3614-72-0) be stored?

4-(Hydrazinomethyl)-1,2,3-benzenetriol (CAS: 3614-72-0) should be stored in a co...

3614-72-04-(Hydrazinomethyl)-...
Compound Q&A

What industries use 4-Amino-1-methyl-1H-pyrazole-5-carboxylic acid hydrochloride (CAS: 92534-70-8)?

4-Amino-1-methyl-1H-pyrazole-5-carboxylic acid hydrochloride (CAS: 92534-70-8) i...

92534-70-84-Amino-1-methyl-1H-...
Compound Q&A

What regulatory guidelines apply to dehydropachymic acid (CAS: 77012-31-8)?

Dehydropachymic acid (CAS: 77012-31-8) is regulated by various agencies. It fall...

77012-31-8Dehydropachymic acid
Compound Q&A

What is the market or research trend for 6-[(2,2-Dimethylpropanoyl)amino]nicotinic acid (CAS: 898561-66-5)?

The market and research trends for 6-[(2,2-Dimethylpropanoyl)amino]nicotinic aci...

898561-66-56-[(2,2-Dimethylprop...
Compound Q&A

How should 1,10-Phenanthroline-2,9-dicarbaldehyde (CAS: 57709-62-3) be stored?

1,10-Phenanthroline-2,9-dicarbaldehyde should be stored in a cool, dry place awa...

57709-62-31,10-Phenanthroline-...
Compound Q&A

How is 5-Carbamoyl-11-oxo-10,11-dihydro-5H-dibenzo[b,f]azepin-10-yl acetate (CAS: 113952-21-9) typically synthesized?

5-Carbamoyl-11-oxo-10,11-dihydro-5H-dibenzo[b,f]azepin-10-yl acetate can be synt...

113952-21-95-Carbamoyl-11-oxo-1...

Source Journal

Physical Chemistry Chemical Physics

Physical Chemistry Chemical Physics
CiteScore: 5.5
Self-citation Rate: 10.3%
Articles per Year: 3036

Physical Chemistry Chemical Physics (PCCP) is an international journal co-owned by 19 physical chemistry and physics societies from around the world. This journal publishes original, cutting-edge research in physical chemistry, chemical physics and biophysical chemistry. To be suitable for publication in PCCP, articles must include significant innovation and/or insight into physical chemistry; this is the most important criterion that reviewers and Editors will judge against when evaluating submissions. The journal has a broad scope and welcomes contributions spanning experiment, theory, computation and data science. Topical coverage includes spectroscopy, dynamics, kinetics, statistical mechanics, thermodynamics, electrochemistry, catalysis, surface science, quantum mechanics, quantum computing and machine learning. Interdisciplinary research areas such as polymers and soft matter, materials, nanoscience, energy, surfaces/interfaces, and biophysical chemistry are welcomed if they demonstrate significant innovation and/or insight into physical chemistry. Joined experimental/theoretical studies are particularly appreciated when complementary and based on up-to-date approaches.

Recommended Compounds

Recommended Suppliers

Disclaimer
This page provides academic journal information for reference and research purposes only. We are not affiliated with any journal publishers and do not handle publication submissions. For publication-related inquiries, please contact the respective journal publishers directly.
If you notice any inaccuracies in the information displayed, please contact us at support@chemtradehub.com. We will promptly review and address your concerns.