Highly selective detection of Cu2+ utilizing specific binding between Cu-demetallated superoxide dismutase 1 and the Cu2+ ion viasurface plasmon resonance spectroscopy

Literature Information

Publication Date 2009-09-11
DOI 10.1039/B910666A
Impact Factor 6.222
Authors

Suseung Lee, Inhee Choi, Surin Hong, Young In Yang, Jeongjin Lee, Taewook Kang, Jongheop Yi


View Original

Abstract

A highly specific interaction between a metal-deficient metalloenzyme and metal ion has been utilized in the selective detection of the metal ion by surface plasmon resonance spectroscopy (SPRS). The use of SPRS and Cu-demetallated superoxide dismutase (E,Zn-SOD1) as a sensing actuator allows one to selectively and in situ detect Cu2+ without any interference that other spectroscopic methods may have.

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