Two-photon microscopy study of the intracellular compartmentalisation of emissive terbium complexes and their oligo-arginine and oligo-guanidinium conjugates

Literature Information

Publication Date 2008-04-22
DOI 10.1039/B803864C
Impact Factor 6.222
Authors

Filip Kielar, Aileen Congreve, Ga-lai Law, Elizabeth J. New, David Parker, Ka-Leung Wong, Pilar Castreňo, Javier de Mendoza


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Abstract

An emissive terbium complex has been conjugated to a C12 chain, Lys-Arg7, Arg7, a tetraguanidinium cation and human serum albumin; two-photon excitation at 720 nm facilitated microscopy studies revealing cell localisation profiles with the oligo-guanidinium conjugate localising in mitochondria but causing apoptotic cell death (IC5012 μM), the C12-amide complex giving rise to necrotic cell death in skin fibroblasts (IC50 8 μM) and the peptide conjugates and the methyl ester generating punctuate cytosolicintracellular distributions.

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