Stereochemistry of hydrogen removal from the ‘unactivated’ C-3 position of 4-hydroxybutyryl-CoA catalysed by 4-hydroxybutyryl-CoA dehydratase

Literature Information

Publication Date 2004-04-23
DOI 10.1039/B402322F
Impact Factor 6.222
Authors

Richard Scott, Ulrike Näser, Peter Friedrich, Thorsten Selmer, Wolfgang Buckel, Bernard T. Golding


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Abstract

(R)- and (S)-γ-[3-2H1]butyrolactones have been synthesised from (R)- and (S)-glycidol, respectively, and used to demonstrate that it is the pro-(S) hydrogen atom that is stereospecifically abstracted from C-3 of 4-hydroxybutyryl-CoA by 4-hydroxybutyryl-CoA dehydratase, and that this atom is not returned to C-4.

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