Metastability and supersaturation limit for lysozyme crystallization
Literature Information
Roberto Piazza
We investigate the ionic strength and temperature dependence of metastability with respect to crystallization in lysozyme solution. The supersaturation limit c* shows a simple scaling relation with salt concentration and, together with the equilibrium solubility csat, approximately factorizes into two terms, respectively accounting for electrostatic and temperature effects. The supersaturation ratio S = c*/csat monotonically increases with the addition of salt. While the dependence of solution properties on the ionic strength is consistent with a recent model by Sear and Warren, temperature effects highlight the importance of hydrophobic contributions.
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